Acousto-optic random-access laser scanning microscopy: Fundamentals and applications to optical recording of neuronal activity

被引：0

作者：

Saggau, P ^{[1
]}

Bullen, A ^{[1
]}

Patel, SS ^{[1
]}

机构：

[1] Baylor Coll Med, Div Neurosci, Houston, TX 77030 USA

来源：

CELLULAR AND MOLECULAR BIOLOGY | 1998年 / 44卷 / 05期

关键词：

laser scanning microscopy; acousto-optic; optical recording; fluorescent probe; nerve cells; review;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel approach to laser scanning microscopy is presented that utilizes diffraction-based scanning principles to achieve fast random-access positioning of a focused laser beam. This non-imaging approach overcomes the speed limitation of present reflection-based scanning microscopes while maintaining high spatial resolution. The presented system combines conventional video microscopy with fast non-imaging scanning microscopy. Together with readily available optical indicators of neuronal activity, this system permits multi-site optical recording from living brain tissue. In this paper, we will review the underlying principles of laser scanning microscopy and the steps in development that led to the current acousto-optic scanning system. We will present typical signals recorded with the current system, and we will outline ongoing extensions of the system. We will also discuss the present limitation of this instrumentation and look into directions of future development.

引用

页码：827 / 846

页数：20