Coherent scattering in multi-harmonic light microscopy

被引：210
作者：
Moreaux, L
Sandre, O
Charpak, S
Blanchard-Desce, M
Mertz, J
机构：
[1] ESPCI, INSERM EPI 00 02, Neurophysiol Lab, F-75005 Paris, France
[2] Inst Curie, Lab Physicochim, CNRS, UMR 168, Paris, France
[3] Univ Rennes, CNRS UMR 6510, Lab Synthese & Electrosynthese Organ, Rennes, France
来源：
BIOPHYSICAL JOURNAL | 2001年 / 80卷 / 03期
关键词：
D O I：
10.1016/S0006-3495(01)76129-2
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
By focusing a pulsed laser beam into a sample, harmonic up-conversion can be generated as well as multi-photon excited fluorescence. Whereas multi-photon excited fluorescence microscopy is well established, the use of multi-harmonic generation for three-dimensional image contrast is very recent. Both techniques can provide similar resolution and, for adequate radiating source density, comparable signal levels, allowing them to be combined in a single versatile instrument.; However, harmonic generation differs fundamentally from fluorescence generation in that it is coherent and produces radiation patterns that are highly sensitive to phase. As such, multi-harmonic generation microscopy provides a unique window into molecular spatial organization that is inaccessible to fluorescence.
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收藏
页码：1568 / 1574
页数：7
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