NOL6 promotes the proliferation and migration of endometrial cancer cells by regulating TWIST1 expression

被引：12

作者：

Liang, Junhui ^{[1
]}

Sun, Wenjing ^{[2
]}

Song, Hui ^{[3
]}

Wang, Chong ^{[4
]}

Li, Qianqian ^{[2
]}

Li, Chunyan ^{[2
]}

Wei, Deying ^{[2
]}

Zhao, Yingzi ^{[2
]}

Li, Changzhong ^{[1
,2
]}

Zhang, Hui ^{[1
,2
]}

机构：

[1] Shandong Univ, Shandong Prov Hosp, Cheeloo Coll Med, Dept Obstet & Gynecol, Jinan 250021, Shandong, Peoples R China

[2] Shandong First Med Univ, Shandong Prov Hosp, Dept Obstet & Gynecol, Jinan 250021, Shandong, Peoples R China

[3] Shandong Univ Tradit Chinese Med, Affiliated Hosp 2, Dept Obstet & Gynecol, Jinan 250001, Shandong, Peoples R China

[4] Shandong Rongjun Gen Hosp, Dept Gen Surg, Jinan 250014, Shandong, Peoples R China

来源：

EPIGENOMICS | 2021年 / 13卷 / 19期

基金：

中国国家自然科学基金;

关键词：

epithelial-mesenchymal transition; NOL6; nucleolar protein; tumorigenesis; TWIST1; NUCLEOLAR PROTEIN; MESENCHYMAL TRANSITION; TUMOR-METASTASIS; GENE; EMT;

D O I：

10.2217/epi-2021-0218

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Lay abstract In endometrial cancer, rapid tumor growth leads to increased protein synthesis and ribosome biogenesis. Our study confirmed the involvement of the protein NOL6 in endometrial cancer. We overexpressed TWIST1, MMP2 or MYC in endometrial cells and assessed the difference in cell growth, spread, death and tumor formation under different conditions. The results showed that NOL6 can boost the growth and spread of endometrial cancer cells by controlling TWIST1 expression. Our study provides a new understanding of the molecular mechanisms causing endometrial cancer. Aim: To investigate the role and function of NOL6, a protein related to ribosome biogenesis, in endometrial cancer. Methods: Methyl thiazolyl tetrazolium assay, colony formation assay, flow cytometry apoptosis assay, transwell and wound healing assays were carried out for evaluating cell proliferation, migration and apoptosis. Immunohistochemistry, western blot and tumor xenograft assays were carried out for detecting the level of protein expression and tumor formation. Results: We demonstrated that NOL6 is overexpressed in endometrial cancer and promotes cell proliferation and migration while reducing apoptosis. NOL6 regulates the expression of TWIST1, which can restore the changes in cells caused by NOL6 knockdown. Conclusions: NOL6 can promote the proliferation and migration of endometrial cancer cells by regulating TWIST1 expression.

引用

页码：1571 / 1585

页数：15

共 32 条

[1]

Andersen JS, 2002, CURR BIOL, V12, P1, DOI 10.1016/S0960-9822(01)00650-9

[2] EMT: 2016 [J].

Angela Nieto, M. ;

Huang, Ruby Yun-Ju ;

Jackson, Rebecca A. ;

Thiery, Jean Paul .

CELL, 2016, 166 (01) :21-45

[3] Nucleolar expansion and elevated protein translation in premature aging [J].

Buchwalter, Abigail ;

Hetzer, Martin W. .

NATURE COMMUNICATIONS, 2017, 8

[4] MEK1 and MEK2 inhibitors and cancer therapy: the long and winding road [J].

Caunt, Christopher J. ;

Sale, Matthew J. ;

Smith, Paul D. ;

Cook, Simon J. .

NATURE REVIEWS CANCER, 2015, 15 (10) :577-592

[5] Validation of nucleolar protein 4 as a novel methylated tumor suppressor gene in head and neck cancer [J].

Demokan, Semra ;

Chuang, Alice Y. ;

Pattani, Kavita M. ;

Sidransky, David ;

Koch, Wayne ;

Califano, Joseph A. .

ONCOLOGY REPORTS, 2014, 31 (02) :1014-1020

[6] NOL6, a new founding oncogene in human prostate cancer and targeted by miR-590-3p [J].

Dong, Degang ;

Song, Mei ;

Wu, Xiaoli ;

Wang, Wanchun .

CYTOTECHNOLOGY, 2020, 72 (03) :469-478

[7] Utp22p acts in concert with Utp8p to channel aminoacyl-tRNA from the nucleolus to the nuclear tRNA export receptor Los1p but not Msn5p [J].

Eswara, Manoja B. K. ;

Clayton, Ashley ;

Mangroo, Dev .

BIOCHEMISTRY AND CELL BIOLOGY, 2012, 90 (06) :731-749

[8]

Fang YY, 2019, ONCOTARGETS THER, V12, P9449, DOI [10.2147/OTT.S181037, 10.2147/OTT.181037]

[9] Nucleolar Proteins Suppress Caenorhabditis elegans Innate Immunity by Inhibiting p53/CEP-1 [J].

Fuhrman, Laura E. ;

Goel, Ajay Kumar ;

Smith, Jason ;

Shianna, Kevin V. ;

Aballay, Alejandro .

PLOS GENETICS, 2009, 5 (09)

[10] Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B [J].

Fujimura, Akiko ;

Hayashi, Yuki ;

Kato, Kazashi ;

Kogure, Yuichiro ;

Kameyama, Mutsuro ;

Shimamoto, Haruka ;

Daitoku, Hiroaki ;

Fukamizu, Akiyoshi ;

Hirota, Toru ;

Kimura, Keiji .

NUCLEIC ACIDS RESEARCH, 2020, 48 (12) :6583-6596

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