Three-dimensional tracking of individual quantum dots

被引:96
作者
Lessard, Guillaume A. [1 ]
Goodwin, Peter M. [1 ]
Werner, James H. [1 ]
机构
[1] Los Alamos Natl Lab, Ctr Integrated Nanotechnol MPA CINT, Los Alamos, NM 87545 USA
关键词
D O I
10.1063/1.2819074
中图分类号
O59 [应用物理学];
学科分类号
摘要
We describe an instrument that extends the state of the art in a single-molecule tracking technology, allowing extended observations of single fluorophores and fluorescently labeled proteins as they undergo directed and diffusive transport in three dimensions. We demonstrate three-dimensional tracking of individual quantum dots undergoing diffusion for durations of over a second at velocities comparable to those of intracellular signaling processes.
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页数:3
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共 24 条
[1]   Single molecule fluorescence spectroscopy at ambient temperature [J].
Ambrose, WP ;
Goodwin, PM ;
Jett, JH ;
Van Orden, A ;
Werner, JH ;
Keller, RA .
CHEMICAL REVIEWS, 1999, 99 (10) :2929-2956
[2]   FLUORESCENT LOW-DENSITY LIPOPROTEIN FOR OBSERVATION OF DYNAMICS OF INDIVIDUAL RECEPTOR COMPLEXES ON CULTURED HUMAN-FIBROBLASTS [J].
BARAK, LS ;
WEBB, WW .
JOURNAL OF CELL BIOLOGY, 1981, 90 (03) :595-604
[3]   HOW TO TRACK BACTERIA [J].
BERG, HC .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1971, 42 (06) :868-&
[4]   Confocal three dimensional tracking of a single nanoparticle with concurrent spectroscopic readouts [J].
Cang, Hu ;
Wong, Chung M. ;
Xu, C. Shan ;
Rizvi, Abbas H. ;
Yang, Haw .
APPLIED PHYSICS LETTERS, 2006, 88 (22)
[5]   Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking [J].
Dahan, M ;
Lévi, S ;
Luccardini, C ;
Rostaing, P ;
Riveau, B ;
Triller, A .
SCIENCE, 2003, 302 (5644) :442-445
[6]   Three-dimensional imaging of single molecules solvated in pores of poly(acrylamide) gels [J].
Dickson, RM ;
Norris, DJ ;
Tzeng, YL ;
Moerner, WE .
SCIENCE, 1996, 274 (5289) :966-969
[7]   Ligand-induced conformational changes observed in single RNA molecules [J].
Ha, T ;
Zhuang, XW ;
Kim, HD ;
Orr, JW ;
Williamson, JR ;
Chu, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (16) :9077-9082
[8]   Nanometric three-dimensional tracking of individual quantum dots in cells [J].
Holtzer, Laurent ;
Meckel, Tobias ;
Schmidt, Thomas .
APPLIED PHYSICS LETTERS, 2007, 90 (05)
[9]   TRACKING OF SINGLE FLUORESCENT PARTICLES IN 3 DIMENSIONS - USE OF CYLINDRICAL OPTICS TO ENCODE PARTICLE POSITION [J].
KAO, HP ;
VERKMAN, AS .
BIOPHYSICAL JOURNAL, 1994, 67 (03) :1291-1300
[10]   CONFINED LATERAL DIFFUSION OF MEMBRANE-RECEPTORS AS STUDIED BY SINGLE-PARTICLE TRACKING (NANOVID MICROSCOPY) - EFFECTS OF CALCIUM-INDUCED DIFFERENTIATION IN CULTURED EPITHELIAL-CELLS [J].
KUSUMI, A ;
SAKO, Y ;
YAMAMOTO, M .
BIOPHYSICAL JOURNAL, 1993, 65 (05) :2021-2040