Production of Barley yellow dwarf virus antisera by DNA immunization
被引:0
作者:
Pal, N
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h-index: 0
机构:
Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USAUniv Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA
Pal, N
[1
]
Moon, JS
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h-index: 0
机构:
Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USAUniv Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA
Moon, JS
[1
]
Sandhu, J
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h-index: 0
机构:
Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USAUniv Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA
Sandhu, J
[1
]
Domier, LL
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h-index: 0
机构:
Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USAUniv Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA
Domier, LL
[1
]
D'Arcy, CJ
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h-index: 0
机构:
Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USAUniv Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA
D'Arcy, CJ
[1
]
机构:
[1] Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA
来源:
CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE
|
2000年
/
22卷
/
04期
基金:
美国农业部;
关键词:
DNA immunization;
BYDV;
ELISA;
CTLA4;
D O I:
10.1080/07060660009500461
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
Antibodies were produced against the 22-kDa coat protein (CP) of Barley yellow dwarf virus strain PAV (BYDV-PAV) by DNA immunization. A cDNA sequence encoding the 22-kDa CP was cloned into a mammalian expression vector (pcDNA22K), entrapped in liposomes, and injected intramuscularly into BALB/c mice. To target the antigen to sites of immune induction and, thereby, enhance the immune response, the 22-kDa sequence was also fused with the sequence encoding the mouse cytotoxic T-lymphocyte antigen 4 to generate pCTLA422K. Three weeks post immunization, BYDV-PAV-specific immune response was detected in serum. Sera from mice injected with pCTLA422K DNA had significantly higher antibody titers than those from mice injected with pcDNA22K. Increases in antibody titers were observed over time with each subsequent DNA injection. Further, there were large increases in antibody titers when DNA-immunized mice were boosted with purified BYDV-PAV virions. These antibody responses were higher than that obtained by a single injection of purified BYDV-PAV. The results demonstrate that antibodies against BYDV-PAV can be generated using DNA immunization. With this approach, it may be possible to produce antisera against luteoviruses that occur in low concentrations in host plant tissue and are very difficult to purify.
机构:
Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
del Blanco, Isabel A.
Hegarty, Joshua
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Hegarty, Joshua
Gallagher, L. W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Gallagher, L. W.
Falk, B. W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Falk, B. W.
Brown-Guedira, G.
论文数: 0引用数: 0
h-index: 0
机构:
NCSU, USDA, ARS, Raleigh, NC 27695 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Brown-Guedira, G.
Pellerin, E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Pellerin, E.
Dubcovsky, J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Howard Hughes Med Inst, Chevy Chase, MD USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Salem, Nida M.
Miller, W. Allen
论文数: 0引用数: 0
h-index: 0
机构:
Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA
Iowa State Univ, Biochem Biophys & Mol Biol Dept, Ames, IA 50011 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Miller, W. Allen
Rowhani, Adib
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Rowhani, Adib
Golino, Deborah A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Golino, Deborah A.
Moyne, Anne-Laure
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Moyne, Anne-Laure
Falk, Bryce W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
机构:
Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
del Blanco, Isabel A.
Hegarty, Joshua
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Hegarty, Joshua
Gallagher, L. W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Gallagher, L. W.
Falk, B. W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Falk, B. W.
Brown-Guedira, G.
论文数: 0引用数: 0
h-index: 0
机构:
NCSU, USDA, ARS, Raleigh, NC 27695 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Brown-Guedira, G.
Pellerin, E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Pellerin, E.
Dubcovsky, J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
Howard Hughes Med Inst, Chevy Chase, MD USAUniv Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Salem, Nida M.
Miller, W. Allen
论文数: 0引用数: 0
h-index: 0
机构:
Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA
Iowa State Univ, Biochem Biophys & Mol Biol Dept, Ames, IA 50011 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Miller, W. Allen
Rowhani, Adib
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Rowhani, Adib
Golino, Deborah A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Golino, Deborah A.
Moyne, Anne-Laure
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
Moyne, Anne-Laure
Falk, Bryce W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USAUniv Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA