Vitamins at physiological levels cause oxidation to the DNA nucleoside deoxyguanosine and to DNA-alone or in synergism with metals

被引:9
作者
Bergstrom, Therese [1 ]
Ersson, Clara [1 ]
Bergman, Jan [1 ]
Moller, Lennart [1 ]
机构
[1] Karolinska Inst, Novum, Dept Biosci & Nutr, SE-14183 Huddinge, Sweden
关键词
ASCORBIC-ACID; BETA-CAROTENE; CARDIOVASCULAR-DISEASE; LUNG-CANCER; HUMAN-PLASMA; DAMAGE; SUPPLEMENTATION; PREVENTION; MORTALITY; SELENIUM;
D O I
10.1093/mutage/ges013
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Vitamins with antioxidant properties have the ability to act as pro-oxidants, inducing oxidative damage and oxidative stress as opposed to preventing it. While vitamin supplements are commonly consumed, the scientific evidence for their health beneficial effects is inconclusive. In fact, even harmful effects have been reported. The present study aimed to investigate and compare pro-oxidant properties of different antioxidants and vitamins commonly found in dietary supplements, at concentrations of physiological relevance, alone or in combination with metals also found in supplements. Focus was on damages related to DNA. The vitamins' chemical oxidation potencies were studied by measuring the amount of the oxidation product 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) formed from the DNA nucleoside deoxyguanosine (dG) after vitamin exposure, using a high-performance liquid chromatography system with electrochemical and ultraviolet detection. To study the vitamins' ability to cause DNA damage to cultured cells, promyelocytic leukemia cells (HL-60) were exposed to vitamins, and strand breaks, alkali-labile sites and oxidative DNA lesions, i.e. formamido pyrimidine DNA glycosylase-sensitive sites, were detected using the comet assay. Vitamins A and C chemically induced oxidation of dG, alone and in synergism with iron or copper, whereas only vitamin C and copper induced DNA damage in cultured cells. Contrary, vitamins B1, B2, B3, B6 and B12, beta-carotene, folic acid, alpha-tocopherol, delta-tocopherol or gamma-tocopherol did not induce oxidative damage to dG, while lycopene induced a weak dose-response increase. Taken together, vitamin C and copper stood out with the strongest oxidative potency, which is of potential concern since both substances are commonly found in multivitamins.
引用
收藏
页码:511 / 517
页数:7
相关论文
共 38 条
  • [1] [Anonymous], 2007, FOOD NUTR PHYS ACT P
  • [2] Dietary Supplement Use in the United States, 2003-2006
    Bailey, Regan L.
    Gahche, Jaime J.
    Lentino, Cindy V.
    Dwyer, Johanna T.
    Engel, Jody S.
    Thomas, Paul R.
    Betz, Joseph M.
    Sempos, Christopher T.
    Picciano, Mary Frances
    [J]. JOURNAL OF NUTRITION, 2011, 141 (02) : 261 - 266
  • [3] Vitamin A and C compounds permitted in supplements differ in their abilities to affect cell viability, DNA and the DNA nucleoside deoxyguanosine
    Bergstrom, Therese
    Bergman, Jan
    Moller, Lennart
    [J]. MUTAGENESIS, 2011, 26 (06) : 735 - 744
  • [4] Mortality in randomized trials of antioxidant supplements for primary and secondary prevention - Systematic review and meta-analysis
    Bjelakovic, Goran
    Nikolova, Dimitrinka
    Gluud, Lise Lotte
    Simonetti, Rosa G.
    Gluud, Christian
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2007, 297 (08): : 842 - 857
  • [5] NUTRITION INTERVENTION TRIALS IN LINXIAN, CHINA - SUPPLEMENTATION WITH SPECIFIC VITAMIN MINERAL COMBINATIONS, CANCER INCIDENCE, AND DISEASE-SPECIFIC MORTALITY IN THE GENERAL-POPULATION
    BLOT, WJ
    LI, JY
    TAYLOR, PR
    GUO, WD
    DAWSEY, S
    WANG, GQ
    YANG, CS
    ZHENG, SF
    GAIL, M
    LI, GY
    YU, Y
    LIU, BQ
    TANGREA, J
    SUN, YH
    LIU, FS
    FRAUMENI, JF
    ZHANG, YH
    LI, B
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1993, 85 (18): : 1483 - 1492
  • [6] Plasma vitamin B6 vitamers before and after oral vitamin B6 treatment:: A randomized placebo-controlled study
    Bor, MV
    Refsum, H
    Bisp, MR
    Bleie, O
    Schneede, J
    Nordrehaug, JE
    Ueland, PM
    Nygard, OK
    Nexo, E
    [J]. CLINICAL CHEMISTRY, 2003, 49 (01) : 155 - 161
  • [7] Brevik A, 2005, AM J CLIN NUTR, V81, P434
  • [8] Therapeutic vitamin A doses increase the levels of markers of oxidative insult in Substantia nigra and decrease locomotory and exploratory activity in rats after acute and chronic supplementation
    de Oliveira, Marcos Roberto
    Silvestrin, Roberta Bristot
    e Souza, Tadeu Mello
    Moreira, Jose Claudio Fonseca
    [J]. NEUROCHEMICAL RESEARCH, 2008, 33 (03) : 378 - 383
  • [9] Vitamin C modulation of H2O2-induced damage and iron homeostasis in human cells
    Duarte, Tiago L.
    Jones, George D. D.
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 2007, 43 (08) : 1165 - 1175
  • [10] Folate, vitamin B12, homocysteine status and DNA damage in young Australian adults
    Fenech, M
    Aitken, C
    Rinaldi, J
    [J]. CARCINOGENESIS, 1998, 19 (07) : 1163 - 1171