Evaluation of Spoligotyping and MIRU-VNTR for Mycobacterium bovis in Xinjiang, China

被引：12

作者：

Sun, Zhaogang ^{[1
]}

Cao, Rui ^{[2
]}

Tian, Miao ^{[1
]}

Zhang, Xuxia ^{[1
]}

Zhang, Xiyue ^{[1
]}

Li, Yan ^{[1
]}

Xu, Yuhui ^{[3
]}

Fan, Weixing

Huang, Baoxu

Li, Chuanyou ^{[1
]}

机构：

[1] Beijing TB & Thorac Tumor Res Inst, Dept Bacteriol & Immunol, Beijing 101149, Peoples R China

[2] Jilin Agr Univ, Coll Anim Sci & Technol, Jilin, Peoples R China

[3] Agr Bur Langfang, Dept Vet Res, Langfang, Hebei, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2012年 / 92卷 / 02期

基金：

中国国家自然科学基金;

关键词：

M; bovis; Cow; Isolation; Spoligotyping; MIRU-VNTR; Epidemic; TUBERCULOSIS COMPLEX; GENETIC DIVERSITY; INFECTIONS; ANIMALS; DIFFERENTIATION; EPIDEMIOLOGY; BACTERIA; STRAINS; REGIONS; HUMANS;

D O I：

10.1016/j.rvsc.2011.03.012

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB) in cattle, is also a pathogen for human and other mammals. In this study, 406 cows were screened for bTB by both single intradermal comparative cervical tuberculin (SICCT) test and IFN-gamma assay. 135 M. bovis were isolated from 31 SICCT and IFN-gamma double-positive cows in Xinjiang Uygur Autonomous Region of China. Spoligotyping and MIRU-VNTR were evaluated for genotyping, and 4 and 7 genotypes were identified, respectively. A new combination of nine MIRU-VNTR loci was most discriminative for M. bovis clones from Xinjiang. Interestingly, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of three loci (ETR-D, QUB 1895 and QUB 3336) were discovered in this study. These results indicated a specific epidemic conservation in Xinjiang, China. M. bovis strains with the unique genotypes were isolated from the herds maintaining parent cows imported from the bTB-free countries, suggesting a possible transmission from the local breed of Xinjiang brown cattle. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：236 / 239

页数：4

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