Ultrastructural study of Chlamydia pneumoniae in a continuous-infection model

被引：41
作者：
Kutlin, A
Flegg, C
Stenzel, D
Reznik, T
Roblin, PM
Mathews, S
Timms, P
Hammerschlag, MR
机构：
[1] Suny Downstate Med Ctr, Dept Pediat, Brooklyn, NY 11203 USA
[2] Queensland Univ Technol, Ctr Mol Biotechnol, Brisbane, Qld 4001, Australia
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 10期
关键词：
D O I：
10.1128/JCM.39.10.3721-3723.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We have established an in vitro model of long-term continuous Chlamydia pneumoniae infection in HEp-2 cells. Using transmission electron microscopy, we demonstrated the presence of spontaneous abnormal chlamydial inclusions similar in appearance to the persistent chlamydial forms induced in vitro by treatment with cytokines or antibiotics or by nutrient deprivation.
引用
收藏
页码：3721 / 3723
页数：3
相关论文
共 20 条
[1]   REACTIVATION OF PERSISTENT CHLAMYDIA-TRACHOMATIS INFECTION IN CELL-CULTURE [J].
BEATTY, WL ;
MORRISON, RP ;
BYRNE, GI .
INFECTION AND IMMUNITY, 1995, 63 (01) :199-205
[2]   TRYPTOPHAN DEPLETION AS A MECHANISM OF GAMMA-INTERFERON-MEDIATED CHLAMYDIAL PERSISTENCE [J].
BEATTY, WL ;
BELANGER, TA ;
DESAI, AA ;
MORRISON, RP ;
BYRNE, GI .
INFECTION AND IMMUNITY, 1994, 62 (09) :3705-3711
[3]   MORPHOLOGIC AND ANTIGENIC CHARACTERIZATION OF INTERFERON GAMMA-MEDIATED PERSISTENT CHLAMYDIA-TRACHOMATIS INFECTION INVITRO [J].
BEATTY, WL ;
BYRNE, GI ;
MORRISON, RP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (09) :3998-4002
[4]   PERSISTENT CHLAMYDIAE - FROM CELL-CULTURE TO A PARADIGM FOR CHLAMYDIAL PATHOGENESIS [J].
BEATTY, WL ;
MORRISON, RP ;
BYRNE, GI .
MICROBIOLOGICAL REVIEWS, 1994, 58 (04) :686-699
[5]   ULTRASTRUCTURAL ANALYSIS OF THE EFFECTS OF ERYTHROMYCIN ON THE MORPHOLOGY AND DEVELOPMENTAL CYCLE OF CHLAMYDIA-TRACHOMATIS HAR-13 [J].
CLARK, RB ;
SCHATZKI, PF ;
DALTON, HP .
ARCHIVES OF MICROBIOLOGY, 1982, 133 (04) :278-282
[6]   ULTRASTRUCTURAL EFFECT OF PENICILLIN AND CYCLOHEXIMIDE ON CHLAMYDIA-TRACHOMATIS STRAIN HAR-13 [J].
CLARK, RB ;
SCHATZKI, PF ;
DALTON, HP .
MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 1982, 171 (03) :151-159
[7]   Persistence of Chlamydia trachomatis is induced by ciprofloxacin and ofloxacin in vitro [J].
Dreses-Werringloer, U ;
Padubrin, I ;
Jürgens-Saathoff, B ;
Hudson, AP ;
Zeidler, H ;
Köhler, L .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (12) :3288-3297
[8]  
GALASSO GJ, 1961, J IMMUNOL, V86, P382
[9]  
Glauert AM, 1998, Biological Specimen Preparation for Transmission Electron Microscopy
[10]   PERSISTENT INFECTION WITH CHLAMYDIA-PNEUMONIAE FOLLOWING ACUTE RESPIRATORY ILLNESS [J].
HAMMERSCHLAG, MR ;
CHIRGWIN, K ;
ROBLIN, PM ;
GELLING, M ;
DUMORNAY, W ;
MANDEL, L ;
SMITH, P ;
SCHACHTER, J .
CLINICAL INFECTIOUS DISEASES, 1992, 14 (01) :178-182
12