The effect of galactose side units and mannan chain length on the macromolecular characteristics of galactomannans

被引：21

作者：

Pitkanen, Leena ^{[1
]}

Tuomainen, Paivi ^{[1
]}

Mikkonen, Kirsi S. ^{[1
]}

Tenkanen, Maija ^{[1
]}

机构：

[1] Univ Helsinki, Dept Food & Environm Sci, FIN-00014 Helsinki, Finland

来源：

CARBOHYDRATE POLYMERS | 2011年 / 86卷 / 03期

关键词：

Galactomannan; Endo-1,4-beta-D-mannanase; alpha-D-galactosidase; Hydrodynamic properties; Size-exclusion chromatography; Field-flow fractionation; GUAR GUM;

D O I：

10.1016/j.carbpol.2011.06.018

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The degree of substitution and the degree of polymerization of galactomannan from guar were altered enzymatically to study the role of side groups and chain length in the behavior of molecules in solution. Unmodified guar galactomannan was found to contain aggregates, but partial removal of galactose side units significantly decreased this tendency. The chain length seemed to dictate the solubility of the molecules together with the degree of substitution. The sample with a relatively low degree of polymerization and low degree of substitution formed large assemblies but stayed in solution while the solubility of the samples with longer chain length was reduced. To our knowledge, this is the first report on the utilization of endo-1,4-beta-D-mannanase and alpha-D-galactosidase as tools for studying the solution properties of galactomannans. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1230 / 1235

页数：6

共 25 条

[1]

BURCHARD W, 2005, POLYSACCHARIDES STRU, P189

[2] Preparation and characterization of molecular weight fractions of guar galactomannans using acid and enzymatic hydrolysis [J].

Cheng, Y ;

Brown, KM ;

Prud'homme, RK .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2002, 31 (1-3) :29-35

[3] On the galactosyl distribution of commercial galactomannans [J].

Daas, PJH ;

Schols, HA ;

de Jongh, HHJ .

CARBOHYDRATE RESEARCH, 2000, 329 (03) :609-619

[4]

Dea I. C. M., 1975, Advances in Carbohydrate Chemistry and Biochemistry, V31, P241

[5] EFFECT OF GALACTOSE-SUBSTITUTION-PATTERNS ON THE INTERACTION PROPERTIES OF GALACTOMANNANS [J].

DEA, ICM ;

CLARK, AH ;

MCCLEARY, BV .

CARBOHYDRATE RESEARCH, 1986, 147 (02) :275-294

[6] Structure of guar in solutions of H2O and D2O:: An ultra-small-angle light-scattering study [J].

Gittings, MR ;

Cipelletti, L ;

Trappe, V ;

Weitz, DA ;

In, M ;

Marques, C .

JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (18) :4381-4386

[7] Optimization of culture conditions for Aspergillus sojae expressing an Aspergillus fumigatus α-galactosidase [J].

Gurkok, Sumeyra ;

Cekmecelioglu, Deniz ;

Ogel, Zumrut B. .

BIORESOURCE TECHNOLOGY, 2011, 102 (07) :4925-4929

[8] Sorption of dissolved galactoglucomannans and galactomannans to bleached kraft pulp [J].

Hannuksela, T ;

Tenkanen, M ;

Holmbom, B .

CELLULOSE, 2002, 9 (3-4) :251-261

[9] RHEOLOGICAL PROPERTIES OF SEED GALACTOMANNAN FROM CASSIA-NODOSA BUCH-HEM [J].

KAPOOR, VP ;

MILAS, M ;

TARAVEL, FR ;

RINAUDO, M .

CARBOHYDRATE POLYMERS, 1994, 25 (02) :79-84

[10]

KOLTERMANN A, 2008, Patent No. 20082223

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