De novo assembly and functional annotation of the transcriptome of Mimachlamys varia, a bioindicator marine bivalve

被引:5
作者
Viricel, Amelia [1 ]
Becquet, Vanessa [1 ]
Dubillot, Emmanuel [1 ]
Pante, Eric [1 ]
机构
[1] Univ La Rochelle, CNRS, UMR 7266, Littoral Environm & Soc LIENSs, 2 Rue Olympe Gouges, F-17042 La Rochelle 01, France
关键词
Pectinidae; de novo assembly; Variegated scallop; Functional annotation; RNA-seq; Biomarker; OXIDATIVE STRESS; BIOMARKERS;
D O I
10.1016/j.margen.2018.04.002
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Developing genomic resources for species used as bioindicators of environmental pollution facilitates identification of new biomarkers of interest. The variegated scallop Mimachlamys varia (Pectinidae) is a marine bivalve used to evaluate and monitor chemical contamination on the French Atlantic coast. Because natural populations of this species are commercially harvested, there is particular interest in understanding its responses to environmental pollution and pathogens. We assembled and annotated the transcriptome of M. varia obtained from a pool of five tissue types (gills, mantle, digestive gland, gonad, adductor muscle). In depth Illumina sequencing led to the assembly of 333,022 transcripts, covering 98% of genes conserved among eukaryotes.
引用
收藏
页码:42 / 45
页数:4
相关论文
共 24 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   Trimmomatic: a flexible trimmer for Illumina sequence data [J].
Bolger, Anthony M. ;
Lohse, Marc ;
Usadel, Bjoern .
BIOINFORMATICS, 2014, 30 (15) :2114-2120
[3]  
Breitwieser M., 2018, J MOLLUSCAN STUD
[4]   First data on three bivalve species exposed to an intra-harbour polymetallic contamination (La Rochelle, France) [J].
Breitwieser, Marine ;
Viricel, Amelia ;
Churlaud, Carine ;
Guillot, Benoit ;
Martin, Elie ;
Stenger, Pierre-Louis ;
Huet, Valerie ;
Fontanaud, Angelique ;
Thomas-Guyon, Helene .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 2017, 199 :28-37
[5]   Short-Term and Long-Term Biological Effects of Chronic Chemical Contamination on Natural Populations of a Marine Bivalve [J].
Breitwieser, Marine ;
Viricel, Amelia ;
Graber, Marianne ;
Murillo, Laurence ;
Becquet, Vanessa ;
Churlaud, Carine ;
Fruitier-Arnaudin, Ingrid ;
Huet, Valerie ;
Lacroix, Camille ;
Pante, Eric ;
Le Floch, Stephane ;
Thomas-Guyon, Helene .
PLOS ONE, 2016, 11 (03)
[6]   Deep sequencing-based transcriptome profiling analysis of Chlamys farreri exposed to benzo[a]pyrene [J].
Cai, Yuefeng ;
Pan, Luqing ;
Hu, Fengxiao ;
Jin, Qian ;
Liu, Tong .
GENE, 2014, 551 (02) :261-270
[7]   InterPro in 2017-beyond protein family and domain annotations [J].
Finn, Robert D. ;
Attwood, Teresa K. ;
Babbitt, Patricia C. ;
Bateman, Alex ;
Bork, Peer ;
Bridge, Alan J. ;
Chang, Hsin-Yu ;
Dosztanyi, Zsuzsanna ;
El-Gebali, Sara ;
Fraser, Matthew ;
Gough, Julian ;
Haft, David ;
Holliday, Gemma L. ;
Huang, Hongzhan ;
Huang, Xiaosong ;
Letunic, Ivica ;
Lopez, Rodrigo ;
Lu, Shennan ;
Marchler-Bauer, Aron ;
Mi, Huaiyu ;
Mistry, Jaina ;
Natale, Darren A. ;
Necci, Marco ;
Nuka, Gift ;
Orengo, Christine A. ;
Park, Youngmi ;
Pesseat, Sebastien ;
Piovesan, Damiano ;
Potter, Simon C. ;
Rawlings, Neil D. ;
Redaschi, Nicole ;
Richardson, Lorna ;
Rivoire, Catherine ;
Sangrador-Vegas, Amaia ;
Sigrist, Christian ;
Sillitoe, Ian ;
Smithers, Ben ;
Squizzato, Silvano ;
Sutton, Granger ;
Thanki, Narmada ;
Thomas, Paul D. ;
Tosatto, Silvio C. E. ;
Wu, Cathy H. ;
Xenarios, Ioannis ;
Yeh, Lai-Su ;
Young, Siew-Yit ;
Mitchell, Alex L. .
NUCLEIC ACIDS RESEARCH, 2017, 45 (D1) :D190-D199
[8]   The use of -omic tools in the study of disease processes in marine bivalve mollusks [J].
Gomez-Chiarri, Marta ;
Guo, Ximing ;
Tanguy, Arnaud ;
He, Yan ;
Proestou, Dina .
JOURNAL OF INVERTEBRATE PATHOLOGY, 2015, 131 :137-154
[9]   Full-length transcriptome assembly from RNA-Seq data without a reference genome [J].
Grabherr, Manfred G. ;
Haas, Brian J. ;
Yassour, Moran ;
Levin, Joshua Z. ;
Thompson, Dawn A. ;
Amit, Ido ;
Adiconis, Xian ;
Fan, Lin ;
Raychowdhury, Raktima ;
Zeng, Qiandong ;
Chen, Zehua ;
Mauceli, Evan ;
Hacohen, Nir ;
Gnirke, Andreas ;
Rhind, Nicholas ;
di Palma, Federica ;
Birren, Bruce W. ;
Nusbaum, Chad ;
Lindblad-Toh, Kerstin ;
Friedman, Nir ;
Regev, Aviv .
NATURE BIOTECHNOLOGY, 2011, 29 (07) :644-U130
[10]  
Grosell Martin, 2006, Oceanography, V19, P126