Regional Differences of Proteins Expressing in Adipose Depots Isolated from Cows, Steers and Bulls as Identified by a Proteomic Approach

被引:9
作者
Cho, Jin Hyoung [2 ,3 ]
Jeong, Jin Young [4 ]
Lee, Ra Ham [2 ,3 ]
Park, Mi Na [4 ]
Kim, Seok-Ho [5 ]
Park, Seon-Min [6 ]
Shin, Jae-Cheon [6 ]
Jeon, Young-Joo [2 ,3 ]
Shim, Jung-Hyun [7 ,8 ]
Choi, Nag-Jin [9 ]
Seo, Kang Seok [10 ]
Cho, Young Sik [11 ]
Kim, MinSeok S. [12 ]
Ko, Sungho [13 ]
Seo, Jae-Min [14 ,15 ,16 ]
Lee, Seung-Youp [17 ,18 ]
Chae, Jung-Il [2 ,3 ]
Lee, Hyun-Jeong [1 ]
机构
[1] Natl Inst Anim Sci, Anim Nutr Physiol Team, Jeonju 565851, South Korea
[2] Chonbuk Natl Univ, Sch Dent, Dept Dent Pharmacol, Jeonju 651756, South Korea
[3] Chonbuk Natl Univ, Inst Dent Biosci, Plus BK21, Jeonju 651756, South Korea
[4] Natl Inst Anim Sci, Anim Prod Utilizat Div, Jeonju 565851, South Korea
[5] Korea Res Inst Biosci & BioTechnol, Aging Res Inst, Daejeon 34141, South Korea
[6] Pohang Ctr Evaluat Biomat, Pohang 37668, South Korea
[7] Mokpo Natl Univ, Dept Pharm, Coll Pharm, Mokpo 534729, South Korea
[8] Mokpo Natl Univ, Nat Med Res Inst, Mokpo 534729, South Korea
[9] Chonbuk Natl Univ, Dept Anim Sci, Coll Agr & Life Sci, Jeonju 54896, South Korea
[10] Sunchon Natl Univ, Dept Anim Sci & Technol, Sunchon 57922, South Korea
[11] Keimyung Univ, Dept Pharm, Daegu 704701, South Korea
[12] Konyang Univ, Dept Biomed Engn, Daejeon 35365, South Korea
[13] CHA Univ, Dept Appl Biosci, Songnam 463836, South Korea
[14] Chonbuk Natl Univ, Sch Dent, Dept Prosthodont, Jeonju 561756, South Korea
[15] Chonbuk Natl Univ, Inst Oral Biosci, Jeonju 561756, South Korea
[16] Chonbuk Natl Univ, Res Inst Clin Med, Jeonju 561756, South Korea
[17] Chonbuk Natl Univ, Inst Oral Biosci, Cluster Craniofacial Dev & Regenerat Res, Jeonju 561756, South Korea
[18] Chonbuk Natl Univ, Sch Dent, Jeonju 561756, South Korea
来源
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2016年 / 29卷 / 08期
关键词
Adipose Depots; Hanwoo; Liquid Chromatography-tandem Mass Spectrometry; Marbling; Meat Quality; INTRAMUSCULAR CONNECTIVE-TISSUE; SKELETAL-MUSCLE; MEAT QUALITY; LONGISSIMUS MUSCLE; FAT DEPOSITION; CATTLE; TENDERNESS; BEEF; DIFFERENTIATION; TROPOMYOSIN;
D O I
10.5713/ajas.16.0051
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Adipose tissue in the loin muscle area of beef cattle as a marbling factor is directly associated with beef quality. To elucidate whether properties of proteins involved in depot specific adipose tissue were sex-dependent, we analyzed protein expression of intramuscular adipose tissue (IMAT) and omental adipose tissue (OMAT) from Hanwoo cows, steers, and bulls of Korean native beef cattle by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis, quantitative polymerase chain reaction (PCR) and western blot analysis. Two different adipose depots (i.e. intramuscular and omental) were collected from cows (n = 7), steers (n = 7), or bulls (n = 7). LC-MS/MS revealed a total of 55 and 35 proteins in IMAT and OMAT, respectively. Of the 55 proteins identified, 44, 40, and 42 proteins were confirmed to be differentially expressed in IMAT of cows, steers, and bulls, respectively. In OMAT of cows, steers, and bulls, 33, 33, and 22 were confirmed to be differentially expressed, respectively. Tropomyosin (TPM) 1, TPM 2, and TPM3 were subjected to verification by quantitative PCR and western blot analysis in IMAT and OMAT of Hanwoo cows, steers, and bulls as key factors closely associated with muscle development. Both mRNA levels and protein levels of TPM1, TPM2, and TPM3 in IMAT were lower in bulls compared to in cows or steers suggesting that they were positively correlated with marbling score and quality grade. Our results may aid the regulation of marbling development and improvement of meat quality grades in beef cattle.
引用
收藏
页码:1197 / 1206
页数:10
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