Ab initio structure determination of a small protein, rubredoxin, by direct methods

被引：13
|
作者：
Mukherjee, M [1 ]
机构：
[1] Indian Assoc Cultivat Sci, Dept Solid State Phys, Kolkata 700032, W Bengal, India
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷
关键词：
D O I：
10.1107/S0907444998016679
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The direct-methods program SAYTAN has been applied successfully to a known protein, rubredoxin. which contains 52 amino-acid residues including an FeS4 unit, a sulfate ion and 102 solvent water molecules. Starting with initially random phases, useful sets can be obtained from multiple trials and selected by figures of merit at different resolutions. Phase extension followed by weighted Fourier recycling reveals a recognizable structure of rubredoxin. The model is refined against 1 Angstrom resolution data to an R factor of 14.5% using the program SHELXL93.
引用
收藏
页码：820 / 825
页数：6
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