Basic Leucine Zipper (bZIP) Domain Transcription Factor MBZ1 Regulates Cell Wall Integrity, Spore Adherence, and Virulence in Metarhizium robertsii

被引:40
作者
Huang, Wei [1 ]
Shang, Yanfang [1 ]
Chen, Peilin [1 ]
Cen, Kai [1 ]
Wang, Chengshu [1 ]
机构
[1] Chinese Acad Sci, Key Lab Insect Dev & Evolutionary Biol, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China
基金
中国国家自然科学基金;
关键词
FUNGAL BIOCONTROL AGENTS; SACCHAROMYCES-CEREVISIAE; ENTOMOPATHOGENIC FUNGUS; CANDIDA-ALBICANS; STRESS RESPONSES; FULL VIRULENCE; GENE; PROTEINS; ADHESION; REVEALS;
D O I
10.1074/jbc.M114.630939
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcription factors (TFs) containing the basic leucine zipper (bZIP) domain are widely distributed in eukaryotes and display an array of distinct functions. In this study, a bZIP-type TF gene (MBZ1) was deleted and functionally characterized in the insect pathogenic fungus Metarhizium robertsii. The deletion mutant (Delta MBZ1) showed defects in cell wall integrity, adhesion to hydrophobic surfaces, and topical infection of insects. Relative to the WT, Delta MBZ1 was also impaired in growth and conidiogenesis. Examination of putative target gene expression indicated that the genes involved in chitin biosynthesis were differentially transcribed in Delta MBZ1 compared with the WT, which led to the accumulation of a higher level of chitin in mutant cell walls. MBZ1 exhibited negative regulation of subtilisin proteases, but positive control of an adhesin gene, which is consistent with the observation of effects on cell autolysis and a reduction in spore adherence to hydrophobic surfaces in Delta MBZ1. Promoter binding assays indicated thatMBZ1can bind to different target genes and suggested the possibility of heterodimer formation to increase the diversity of the MBZ1 regulatory network. The results of this study advance our understanding of the divergence of bZIP-type TFs at both intra-and interspecific levels.
引用
收藏
页码:8218 / 8231
页数:14
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