Antiproliferative activity of Luehea candicans Mart. et Zucc. (Tiliaceae)

被引：11

作者：

da Silva, Dioni A. ^{[1
]}

Alves, Vanessa G. ^{[1
]}

Franco, Danielle M. M. ^{[3
]}

Ribeiro, Laryssa C. ^{[3
]}

de Souza, Maria C. ^{[2
]}

Kato, Lucilia ^{[3
]}

de Carvalho, Joao E. ^{[4
]}

Kohn, Luciana K. ^{[4
]}

de Oliveira, Cecilia M. A. ^{[3
]}

da Silva, Cleuza C. ^{[1
]}

机构：

[1] Univ Estadual Maringa, Dept Quim, BR-87020900 Maringa, Parana, Brazil

[2] Univ Estadual Maringa, Nucleo Pesquisas Limnol Ictiol & Aquicultura, BR-87020900 Maringa, Parana, Brazil

[3] Univ Fed Goias, Inst Quim, BR-74001970 Goiania, Go, Brazil

[4] Ctr Pluridisciplinar Pesquisas Quim Biol & Agr 61, BR-13083970 Campinas, SP, Brazil

来源：

NATURAL PRODUCT RESEARCH | 2012年 / 26卷 / 04期

关键词：

Luehea; antiproliferative activity; lupeol; CONSTITUENTS; TRITERPENOIDS; DERIVATIVES; INHIBITORS; SPECTRA; NMR;

D O I：

10.1080/14786411003752102

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Luehea candicans Mart. et Zucc. (Tiliaceae) is known as 'acoita-cavalo' and is one of the most important medicinal plants found in the Brazilian cerrado. The crude methanolic extracts of the branches and leaves and their fractions were evaluated using the following cancer cell lines: MCF-7 (breast), NCI-ADR (breast expressing the multidrug resistance phenotype), NCI-460 (lung), UACC-62 (melanoma), 786-0 (kidney), OVCAR (ovarian), PCO-3 (prostate), HT-29 (colon) and K-562 (leukaemia). The crude methanolic extracts from the branches (B) and leaves (L) were able to inhibit the growth of the K-562 and 786-0 cell lines in a dose-dependent manner, with GI(50) values of 8.1 and 5.4 mu g mL(-1), respectively. The hexane (L1), chloroform (L2) and methanol (L4) fractions derived from extract L showed a high selectivity and pronounced cytostatic activity against 786-0 (GI(50) similar to 40 mu g mL(-1)). A significant amount of lupeol was isolated from fraction L2. The chloroform (B2) and methanol (B3) fractions derived from extract (B) exhibited less selectivity, showing the highest cytostatic activity against K-562, NCI-ADR, OVCAR, MCF-7 and NCI-460 cells, with GI50 values between 27 and 40 mu g mL(-1). Lupeol, betulin, a mixture of steroids, (-)-epicatechin, vitexin and liriodendrin were isolated from these active fractions.

引用

页码：364 / 369

页数：6

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