Accumulation of focal adhesion protein hic-5 in the nucleus by hydrogen peroxide

被引：3

作者：

Shibanuma, M ^{[1
]}

Ishino, K ^{[1
]}

Sakamoto, N ^{[1
]}

Nose, K ^{[1
]}

机构：

[1] Showa Univ, Sch Pharmaceut Sci, Dept Microbiol, Shinagawa Ku, Tokyo 1428555, Japan

来源：

ACTA HISTOCHEMICA ET CYTOCHEMICA | 2001年 / 34卷 / 04期

关键词：

hic-5; nuclear translocation; NES; hydrogen peroxide;

D O I：

10.1267/ahc.34.259

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Reactive oxygen species (ROS) are produced in cultured cells upon stimulation with cytokines and tumor promoters, and participate in signal transduction pathways leading to gene expression. The hic-5 gene was isolated as a hydrogen peroxide-inducible clone by differential screening, and was shown to encode a paxillin-related protein localized in the focal adhesion plaques. In mouse osteoblastic and fibroblastic cells treated with low concentrations of hydrogen peroxide, Hic-5 protein was found to be reversibly localized to the nucleus within 30 min. Hic-5 was also translocated to the nucleus in cells treated with leptomycin B, an inhibitor of nuclear export of proteins. Hic-5 protein with mutations in the nuclear export signal and the LIM-3 domain accumulated in the nucleus. These results indicated that Hic-5 shuttles between the cytoplasm and the nucleus, and may participate both in surface signals and in gene expression.

引用

页码：259 / 264

页数：6