Human cytomegalovirus miR-US5-1 inhibits viral replication by targeting Geminin mRNA

被引：12

作者：

Jiang, Shujuan ^{[1
,2
]}

Huang, Yujing ^{[1
]}

Qi, Ying ^{[1
]}

He, Rong ^{[2
]}

Liu, Zhongyang ^{[1
]}

Ma, Yanping ^{[1
]}

Guo, Xin ^{[1
]}

Shao, Yaozhong ^{[1
]}

Sun, Zhengrong ^{[1
]}

Ruan, Qiang ^{[1
]}

机构：

[1] China Med Univ, Affiliated Shengjing Hosp, Virus Lab, Shenyang 110122, Liaoning, Peoples R China

[2] China Med Univ, Affiliated Shengjing Hosp, Clin Genet, Shenyang 110122, Liaoning, Peoples R China

来源：

VIROLOGICA SINICA | 2017年 / 32卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Human cytomegalovirus (HCMV); hcmv-miR-US5-1; Geminin (GMNN); DNA replication; cell cycle; DNA-REPLICATION; PROLIFERATION; EXPRESSION; MICRORNA; INFECTION; MECHANISMS; RESPONSES; CELLS; CDT1;

D O I：

10.1007/s12250-017-4064-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Viruses commonly create favorable cellular conditions for their survival through multiple mechanisms. MicroRNAs (miRNAs), which function as post-transcriptional regulators, are utilized by human cytomegalovirus (HCMV) in its infection and pathogenesis. In the present study, the DNA replication inhibitor Geminin (GMNN) was identified to be a direct target of hcmv-miR-US5-1. Overexpression of hcmv-miR-US5-1 could block the accumulation of GMNN during HCMV infection, and the decrease of GMNN expression caused by hcmv-miR-US5-1 or GMNN specific siRNA reduced HCMV DNA copies in U373 cells. Meanwhile, ectopic expression of hcmv-miR-US5-1 and consequent lower expression of GMNN influenced host cell cycle and proliferation. These results imply that hcmv-miR-US5-1 may affect viral replication and host cellular environment by regulating expression kinetics of GMNN during HCMV infection.

引用

页码：431 / 439

页数：9

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