Detection and Identification of Three 'Candidatus Phytoplasma' Species in Picea spp. Trees in Poland

被引:5
作者
Kaminska, Maria [1 ]
Berniak, Hanna [1 ]
机构
[1] Inst Hort, Dept Plant Protect, PL-96100 Skierniewice, Poland
关键词
spruce; phytoplasma; molecular detection; Poland; PINI;
D O I
10.1111/j.1439-0434.2011.01842.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We report the detection of phytoplasmas in Picea abies, Picea glauca and Picea pungens trees with witches' brooms and other growth abnormalities and also in symptomless trees. Phytoplasmas were detected in c. 25% of the tested plants by polymerase chain reaction using phytoplasma universal P1/P7 followed by R16F2n/R16R2 primer pairs. The phytoplasmas were classified as members of the phylogenetic groups: 16SrI, 'Ca. Phytoplasma asteris'; 16SrIII, X-disease phytoplasma group; and 16SrXXI, 'Ca. Phytoplasma pini'.
引用
收藏
页码:796 / 798
页数:3
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