Non-covalent and covalent immobilization of Candida antarctica lipase B on chemically modified multiwalled carbon nanotubes for a green acylation process in supercritical CO2

被引:36
作者
Bourkaib, Mohamed Chafik [1 ]
Guiavarc'h, Yann [1 ]
Chevalot, Isabelle [1 ]
Delaunay, Stephane [1 ]
Gleize, Jerome [2 ]
Ghanbaja, Jaafar [3 ]
Valsaque, Fabrice [3 ]
Berrada, Nawal [3 ]
Desforges, Alexandre [3 ]
Vigolo, Brigitte [3 ]
机构
[1] Univ Lorraine, Lab React & Genie Proc, CNRS, F-54000 Nancy, France
[2] Univ Lorraine, Lab Chim Phys Approche Multiechelle Milieux Compl, F-57078 Metz, France
[3] Univ Lorraine, Inst Jean Lamour, CNRS, F-54000 Nancy, France
关键词
Lipase immobilization; Carbon nanotubes; Supercritical CO2; Green solvent; Interactions; ENZYMATIC ACYLATION; FUNCTIONALIZATION; ADSORPTION; PURIFICATION; SELECTIVITY; OXIDATION; ENZYMES; RUGOSA; MEDIA;
D O I
10.1016/j.cattod.2019.08.046
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Candida antarctica B lipase (CAL-B) was immobilized on purified and functionalized multiwalled carbon nanotubes (MWCNTs). Both immobilization routes, physical adsorption and covalent bonding, were investigated. MWCNT functionalization by a non-aggressive oxidation by potassium permanganate led to an interesting balance between the hydrophilic and the hydrophobic areas of the MWCNT surface; the former being responsible of the good dispersion of MWCNTs in water and the latter having a favorable affinity with CAL-B. The enzyme loadings reached were significant: around 16 wt. % and 21 wt.% for non-covalent and covalent immobilization, respectively. The enzymatic activity was studied with the reaction of O-acylation of geraniol into geranyl acetate by CAL-B in supercritical CO2. Even if a decay in synthesis of geranyl acetate was observed over cycling for both CAL-B@MWCNT catalysts, it was demonstrated that the regioselectivity of CAL-B was unchanged through immobilization on the MWCNT surface for both routes. Interestingly, it was shown that a fully green enzymatic process can be achieved with these prepared CAL-B@MWCNT biocatalyst. Such approach could be transferred to other support/enzyme systems for developing new eco-friendly synthesis processes.
引用
收藏
页码:26 / 36
页数:11
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