Detection of streptomycin residues in whole milk using an optical immunobiosensor

被引:70
作者
Baxter, GA [1 ]
Ferguson, JP [1 ]
O'Connor, MC [1 ]
Elliott, CT [1 ]
机构
[1] Dept Agr & Rural Dev, Vet Sci Div, Belfast BT4 3SD, Antrim, North Ireland
关键词
Biosensor; streptomycin; drug residues; milk;
D O I
10.1021/jf001484l
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The development of an assay for the detection of streptomycin residues in pasteurized whole milk using an optical biosensor (Biacore) is reported. Streptomycin-adipic hydrazide coupled to bovine thyroglobulin was used to produce a sheep polyclonal antibody. The antibody displayed excellent cross-reactivity with dihydrostreptomycin (106%). There was no significant cross-reaction with other aminoglycosides or common antibiotics. Streptomycin was also immobilized onto a CM5 sensor chip to provide a stable, reusable surface. The developed assay permitted the direct analysis of whole milk samples (similar to3.5% fat) without prior centrifugation and defatting. Results were available in 5 min. The limit of detection of the assay was determined as 4.1 ng/mL, well below the European maximum residue limit (MRL) of 200 ng/mL. Repeatability (or coefficient of variation) between runs was determined as 3.5% (100 ng/mL; 0.5 x MRL), 5.7% (200 ng/mL; MRL), and 7.6% (400 ng/mL; 2 x MRL).
引用
收藏
页码:3204 / 3207
页数:4
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