B cell receptor-mediated nuclear fragmentation proceeds in WEHI 231 cells in the absence of detectable DEVDase and FRase activity

被引:13
作者
Mlinaric-Rascan, I
Turk, B
机构
[1] Univ Ljubljana, Fac Pharm, Ljubljana 1000, Slovenia
[2] Josef Stefan Inst, Dept Biochem & Mol Biol, Ljubljana 1000, Slovenia
关键词
caspase; cathepsin; B cell receptor; apoptosis; inhibitor;
D O I
10.1016/S0014-5793(03)00966-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Crosslinking of the WEHI 231 lymphoma B cell receptor (BCR) leads to growth arrest followed by apoptosis. In a study of the role of lysosomal cysteine proteinases in BCR-mediated apoptosis we provide evidence that commitment to apoptosis correlates with a time-dependent increase in caspase and cathepsin activities. We also show that activation of cathepsins is a caspase-independent process, and caspase cascade activation is independent of lysosomal endopeptidases. BCR-induced nuclear fragmentation was not prevented, but rather delayed in the absence of detectable caspase and cathepsin activities, suggesting that BCR-driven apoptosis of these cells may use an alternative proteolytic mechanism independent of caspases and cathepsins. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:51 / 55
页数:5
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