Refining hot-water extracted silk sericin by ethanol-induced precipitation

被引：60

作者：

Oh, Hanjin ^{[1
]}

Lee, Ji Young ^{[2
]}

Kim, Moo Kon ^{[1
]}

Um, In Chul ^{[3
]}

Lee, Ki Hoon ^{[1
,4
]}

机构：

[1] Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151921, South Korea

[2] Seoul Natl Univ, Natl Instrumentat Ctr Environm Management, Seoul 151921, South Korea

[3] Kyungpook Natl Univ, Dept Nat Fiber Sci, Taegu 702701, South Korea

[4] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 151921, South Korea

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2011年 / 48卷 / 01期

关键词：

Silk; Sericin; Bombyx mori; Protein-based material; INDUSTRY WASTE-WATER; BOMBYX-MORI; PROTEIN; FIBROIN; POLYPEPTIDES; SPECTROSCOPY; COMPONENTS; PEPTIDES; FIBERS; COCOON;

D O I：

10.1016/j.ijbiomac.2010.09.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to improve some inherent disadvantages in the mechanical properties of silk sericin (SS), we prepared ethanol-precipitated sericin (EpSS) by adding ethanol into hot-water extracted sericin (HS) solution. EpSS had higher viscosity compared to HS and it was due to the differences in their molecular weight distribution (MWD). The different MWD of EpSS was due to the different solubility of sericin molecules at various concentrations of ethanol, and the amino acid composition of EpSS showed that the more hydrophobic sericin is precipitated more readily in the presence of ethanol. The secondary structure of sericin is also changed from a random coil to beta-sheet structure when the amount of ethanol added is high enough. The DSC analysis also revealed that EpSSs has a more compact structure. Finally, when beads were prepared from EpSS, they had enhanced compressive strength compared to those from HS. (C) 2010 Elsevier ay. All rights reserved.

引用

页码：32 / 37

页数：6

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