Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS

被引：115

作者：

Lembcke, J ^{[1
]}

Ceglarek, U ^{[1
]}

Fiedler, GM ^{[1
]}

Baumann, S ^{[1
]}

Leichtle, A ^{[1
]}

Thiery, J ^{[1
]}

机构：

[1] Univ Hosp, Inst Lab Med Clin Chem & Mol Diagnost, Leipzig, Germany

来源：

JOURNAL OF LIPID RESEARCH | 2005年 / 46卷 / 01期

关键词：

free phytosterols; phytosoterol esters; liquid chromatography; plant sterols;

D O I：

10.1194/jlr.C400004-JLR200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel analytical platform based on liquid chromatography and tandem mass spectrometry using atmospheric pressure photoionization was applied for the simultaneous quantification of free and esterified P-sitosterol, campesterol, brassicasterol, and stigmasterol. The total time for sample pretreatment and analysis could be reduced from similar to3 h [gas chromatography-mass spectrometry (GC-MS)] to 15 min. The detection limits of the different phytosterols; ranged between 0.25 and 0.68 mug/l. Linear ranges were between 1 and 1,000 mug/l. The within-run and between-rim variabilities ranged between 1.4% and 9.9%. The analytical sensitivity was at least 150-fold higher compared with GC-MS. Our new method allows a rapid and simultaneous determination of free and esterified phytosterols in serum.

引用

页码：21 / 26

页数：6

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