The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and β2-microglobulin-free HLA-G molecules

被引:97
作者
Gonen-Gross, T
Achdout, H
Arnon, TI
Gazit, R
Stern, N
Horejsí, V
Goldman-Wohl, D
Yagel, S
Mandelboim, O [1 ]
机构
[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Lautenberg Ctr Gen & Tumor Immunol, IL-91120 Jerusalem, Israel
[2] Acad Sci Czech Republ, Inst Mol Genet, Prague, Czech Republic
[3] Hadassah Univ Hosp, Dept Obstet & Gynecol, IL-91120 Jerusalem, Israel
关键词
D O I
10.4049/jimmunol.175.8.4866
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
For a proper development of the placenta, maternal NK cells should not attack the fetal extravillous cytotrophoblast cells. This inhibition of maternal NK cells is partially mediated via the nonclassical MHC class I molecule HLA-G. Recently, we demonstrated that HLA-G forms disulfide-linked high molecular complexes on the surface of transfected cells. In the present study, we demonstrate that HLA-G must associate with beta(2)m for its interaction with CD85J/leukocyte Ig-like receptor-1 (LIR-1). Although HLA-G free H chain complexes are expressed on the surface, they are not recognized and possibly interfere with CD85J/LIR-1 and HLA-G interaction. The formation of these complexes on the cell surface might represent a novel mechanism developed specifically by the HLA-G protein aimed to control the efficiency of the CD85J/LIR-1-mediated inhibition. We also show that endogenous HLA-G complexes are expressed on the cell surface. These findings provide novel insights into the delicate interaction between extravillous cytotrophoblast cells and NK cells in the decidua.
引用
收藏
页码:4866 / 4874
页数:9
相关论文
共 68 条
[1]   Cutting edge: Leukocyte receptor complex-encoded immunomodulatory receptors show differing specificity for alternative HLA-B27 structures [J].
Allen, RL ;
Raine, T ;
Haude, A ;
Trowsdale, J ;
Wilson, MJ .
JOURNAL OF IMMUNOLOGY, 2001, 167 (10) :5543-5547
[2]   Formation of HLA-B27 homodimers and their relationship to assembly kinetics [J].
Antoniou, AN ;
Ford, S ;
Taurog, JD ;
Butcher, GW ;
Powis, SJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (10) :8895-8902
[3]  
Avril T, 1999, J IMMUNOL, V162, P5902
[4]   HLA-G remains a mystery [J].
Bainbridge, D ;
Ellis, S ;
Le Bouteiller, P ;
Sargent, I .
TRENDS IN IMMUNOLOGY, 2001, 22 (10) :548-552
[5]   Lymphoblastoid cells express HLA-1327 homodimers both intracellularly and at the cell surface following endosomal recycling [J].
Bird, LA ;
Peh, CA ;
Kolinberger, S ;
Elliott, T ;
McMichael, AJ ;
Bowness, P .
EUROPEAN JOURNAL OF IMMUNOLOGY, 2003, 33 (03) :748-759
[6]   Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: A comparative study [J].
Blaschitz, A ;
Hutter, H ;
Leitner, V ;
Pilz, S ;
Wintersteiger, R ;
Dohr, G ;
Sedlmayr, P .
HUMAN IMMUNOLOGY, 2000, 61 (11) :1074-1085
[7]   Disulfide bond-mediated dimerization of HLA-G on the cell surface [J].
Boyson, JE ;
Erskine, R ;
Whitman, MC ;
Chiu, M ;
Lau, JM ;
Koopman, LA ;
Valter, MM ;
Angelisova, P ;
Horejsi, V ;
Strominger, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (25) :16180-16185
[8]   CD1d and invariant NKT cells at the human maternal-fetal interface [J].
Boyson, JE ;
Rybalov, B ;
Koopman, LA ;
Exley, M ;
Balk, SP ;
Racke, FK ;
Schatz, F ;
Masch, R ;
Wilson, SB ;
Strominger, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) :13741-13746
[9]   Progress of HLA-G in cancer [J].
Carosella, ED ;
Dausset, J .
SEMINARS IN CANCER BIOLOGY, 2003, 13 (05) :315-316
[10]   HLA-G revisited [J].
Carosella, ED ;
Dausset, J ;
Kirszenbaum, M .
IMMUNOLOGY TODAY, 1996, 17 (09) :407-409