A graphene-based platform for induced pluripotent stem cells culture and differentiation

被引:390
作者
Chen, G. -Y. [1 ]
Pang, D. W. -P. [1 ]
Hwang, S. -M. [2 ]
Tuan, H. -Y. [1 ]
Hu, Y. -C. [1 ]
机构
[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 300, Taiwan
[2] Food Ind Res & Dev Inst, Bioresource Collect & Res Ctr, Hsinchu 300, Taiwan
关键词
Graphene; Graphene oxide; Induced pluripotent stem cells; Differentiation; Proliferation; Stem cells; FIBROBLASTS; BACULOVIRUS; VECTOR;
D O I
10.1016/j.biomaterials.2011.09.071
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Induced pluripotent stem cells (iPSCs) hold great promise as a cell source for regenerative medicine yet its culture, maintenance of pluripotency and induction of differentiation remain challenging. Conversely, graphene (G) and graphene oxide (GO) have captured tremendous interests in the fields of materials science, physics, chemistry and nanotechnology. Here we report on that G and GO can support the mouse iPSCs culture and allow for spontaneous differentiation. Intriguingly, G and GO surfaces led to distinct cell proliferation and differentiation characteristics. In comparison with the glass surface, iPSCs cultured on the G surface exhibited similar degrees of cell adhesion and proliferation while iPSCs on the GO surface adhered and proliferated at a faster rate. Moreover, G favorably maintained the iPSCs in the undifferentiated state while GO expedited the differentiation. The iPSCs cultured on both G and GO surfaces spontaneously differentiated into ectodermal and mesodermal lineages without significant disparity, but G suppressed the iPSCs differentiation towards the endodermal lineage whereas GO augmented the endodermal differentiation. These data collectively demonstrated that the different surface properties of G and GO governed the iPSCs behavior and implicate the potentials of graphene-based materials as a platform for iPSCs culture and diverse applications. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:418 / 427
页数:10
相关论文
共 36 条
[1]   Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes [J].
Akasaka, Tsukasa ;
Yokoyama, Atsuro ;
Matsuoka, Makoto ;
Hashimoto, Takeshi ;
Watari, Fumio .
CARBON, 2011, 49 (07) :2287-2299
[2]   Ultrasensitive electrochemical immunoassay for BRCA1 using BMIM•BF4-coated SBA-15 as labels and functionalized graphene as enhancer [J].
Cai, Yanyan ;
Li, He ;
Du, Bin ;
Yang, Minghui ;
Li, Yan ;
Wu, Dan ;
Zhao, Yanfang ;
Dai, Yuxue ;
Wei, Qin .
BIOMATERIALS, 2011, 32 (08) :2117-2123
[3]   In vitro toxicity evaluation of graphene oxide on A549 cells [J].
Chang, Yanli ;
Yang, Sheng-Tao ;
Liu, Jia-Hui ;
Dong, Erya ;
Wang, Yanwen ;
Cao, Aoneng ;
Liu, Yuanfang ;
Wang, Haifang .
TOXICOLOGY LETTERS, 2011, 200 (03) :201-210
[4]   Carbon nanotubes promote neuron differentiation from human embryonic stem cells [J].
Chao, Tzu-I ;
Xiang, Shuhuai ;
Chen, Chi-Shuo ;
Chin, Wei-Chun ;
Nelson, A. J. ;
Wang, Changchun ;
Lu, Jennifer .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 384 (04) :426-430
[5]   A graphene functionalized electrochemical aptasensor for selective label-free detection of cancer cells [J].
Feng, Lingyan ;
Chen, Yong ;
Ren, Jinsong ;
Qu, Xiaogang .
BIOMATERIALS, 2011, 32 (11) :2930-2937
[6]   Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin [J].
Hanna, Jacob ;
Wernig, Marius ;
Markoulaki, Styliani ;
Sun, Chiao-Wang ;
Meissner, Alexander ;
Cassady, John P. ;
Beard, Caroline ;
Brambrink, Tobias ;
Wu, Li-Chen ;
Townes, Tim M. ;
Jaenisch, Rudolf .
SCIENCE, 2007, 318 (5858) :1920-1923
[7]   The control of neural cell-to-cell interactions through non-contact electrical field stimulation using graphene electrodes [J].
Heo, Chaejeong ;
Yoo, Jeongwan ;
Lee, Siyoung ;
Jo, Areum ;
Jung, Susie ;
Yoo, Hyosun ;
Lee, Young Hee ;
Suh, Minah .
BIOMATERIALS, 2011, 32 (01) :19-27
[8]   Baculovirus transduction of human mesenchymal stem cell-derived progenitor cells: variation of transgene expression with cellular differentiation states [J].
Ho, Y-C ;
Lee, H-P ;
Hwang, S-M ;
Lo, W-H ;
Chen, H-C ;
Chung, C-K ;
Hu, Y-C .
GENE THERAPY, 2006, 13 (20) :1471-1479
[9]   Protein Corona-Mediated Mitigation of Cytotoxicity of Graphene Oxide [J].
Hu, Wenbing ;
Peng, Cheng ;
Lv, Min ;
Li, Xiaoming ;
Zhang, Yujie ;
Chen, Nan ;
Fan, Chunhai ;
Huang, Qing .
ACS NANO, 2011, 5 (05) :3693-3700
[10]   Efficient gene transfer into mouse embryonic stem cells with adenovirus vectors [J].
Kawabata, K ;
Sakurai, F ;
Yamaguchi, T ;
Hayakawa, T ;
Mizuguchi, H .
MOLECULAR THERAPY, 2005, 12 (03) :547-554