Evolving strategies for enzyme engineering

被引：276
作者：
Bloom, JD
Meyer, MM
Meinhold, P
Otey, CR
MacMillan, D
Arnold, FH
机构：
[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
[2] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2005年 / 15卷 / 04期
关键词：
D O I：
10.1016/j.sbi.2005.06.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Directed evolution is a common technique to engineer enzymes for a diverse set of applications. Structural information and an understanding of how proteins respond to mutation and recombination are being used to develop improved directed evolution strategies by increasing the probability that mutant sequences have the desired properties. Strategies that target mutagenesis to particular regions of a protein or use recombination to introduce large sequence changes can complement full-gene random mutagenesis and pave the way to achieving ever more ambitious enzyme engineering goals.
引用
收藏
页码：447 / 452
页数：6
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