Molecular cloning, mapping, and characterization of a novel human gene, MTA1-L1, showing homology to a metastasis-associated gene, MTA1

被引：35

作者：

Futamura, M

Nishimori, H

Shiratsuchi, T

Saji, S

Nakamura, Y

Tokino, T

机构：

[1] Univ Tokyo, Inst Med Sci, Ctr Human Genome, Mol Med Lab,Minato Ku, Tokyo 1088639, Japan

[2] Gifu Univ, Dept Surg 2, Gifu, Japan

来源：

JOURNAL OF HUMAN GENETICS | 1999年 / 44卷 / 01期

关键词：

human genome project; cDNA library screening; cancer; metastasis; MTA1;

D O I：

10.1007/s100380050107

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Through large-scale sequencing of clones randomly selected from libraries of human cDNAs, we have isolated a novel human gene encoding a product with 59.6% identity in amino acid sequence to human MTA1, a protein associated with tumor invasion and metastasis. This cDNA, named MTA1-L1 (MTA1 like 1), consists of 2736 nucleotides with an open reading frame encoding 668 amino acids. A single 3.0-kb transcript of MTA1-L1 was expressed ubiquitously on Northern blots. Structural analysis of the MTA1-L1 gene revealed 18 exons spanning 8.1 kb of genomic DNA. We assigned the MTA1-L1 locus to chromosomal band 11q12-13.1 by fluorescence in situ hybridization.

引用

页码：52 / 56

页数：5

共 12 条

[1] HIGH-RESOLUTION CYTOGENETIC MAPPING OF 342 NEW COSMID MARKERS INCLUDING 43 RFLP MARKERS ON HUMAN CHROMOSOME-17 BY FLUORESCENCE IN-SITU HYBRIDIZATION [J].