Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Bread Wheat (Triticum aestivum L.)

被引:44
作者
Zhan, Haoshuang [1 ,2 ]
Yue, Hong [1 ,2 ]
Zhao, Xian [1 ,2 ]
Wang, Meng [1 ,2 ]
Song, Weining [1 ,2 ,3 ]
Nie, Xiaojun [1 ,2 ]
机构
[1] Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Peoples R China
[2] Northwest A&F Univ, Yangling Branch, China Wheat Improvement Ctr, Yangling 712100, Peoples R China
[3] Australia China Joint Res Ctr Abiot & Biot Stress, Yangling 712100, Peoples R China
关键词
abiotic stress; co-expression network; expression profiles; Triticum aestivum; EXPRESSION ANALYSIS; MULTIPLE STRESSES; PROTEIN; SALT; OVEREXPRESSION; TOLERANCE; DROUGHT; NETWORK;
D O I
10.3390/genes8100284
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The mitogen-activated protein kinase (MAPK) cascade is a universal signal transduction module that plays a vital role in regulating growth and development, as well as environmental stress responses in plants. Wheat is one of the most important crops worldwide. Although the MAPK kinase kinase (MAP3K) family in wheat has been investigated, the MAPK and MAPK kinase (MAP2K) gene families remain unknown at present. Here, 54 MAPK and 18 MAPKK genes were identified in wheat using recent genomic information. Phylogenetic analysis of Triticum aestivum L. MAPKs and MAPKKs (TaMAPKs and TaMAPKKs) together with homologous genes from other species classified them into four groups, and the clustering was consistent with the genomic exon/intron structures. Conserved motif analysis found that MAPK proteins contained a typical TXY phosphorylation site and MAPKK proteins contained an S/T-X5-S/T motif. RNA-seq data mapping analysis showed that MAPK and MAPKK genes in group IV had tissue-specific expression profiles, whereas each group I member showed relatively high expression in all organs. Expression patterns of TaMAPK and TaMAPKK genes under stress conditions were also investigated and stress-responsive candidates were identified. Co-expression network analysis identified 11 TaMAPK genes and 6 TaMAPKK genes involved in the interaction network pathway. Overall, this study provided useful information for evolutionary and functional surveys of MAPK and MAPKK gene families in wheat and beyond.
引用
收藏
页数:11
相关论文
共 31 条
[1]   Molecular characterization of the Salicornia brachiata SbMAPKK gene and its expression by abiotic stress [J].
Agarwal, Pradeep K. ;
Gupta, Kapil ;
Jha, Bhavanath .
MOLECULAR BIOLOGY REPORTS, 2010, 37 (02) :981-986
[2]   MEME SUITE: tools for motif discovery and searching [J].
Bailey, Timothy L. ;
Boden, Mikael ;
Buske, Fabian A. ;
Frith, Martin ;
Grant, Charles E. ;
Clementi, Luca ;
Ren, Jingyuan ;
Li, Wilfred W. ;
Noble, William S. .
NUCLEIC ACIDS RESEARCH, 2009, 37 :W202-W208
[3]   Triticeae Resources in Ensembl Plants [J].
Bolser, Dan M. ;
Kerhornou, Arnaud ;
Walts, Brandon ;
Kersey, Paul .
PLANT AND CELL PHYSIOLOGY, 2015, 56 (01) :e3
[4]   Conditional Ablation of Ezh2 in Murine Hearts Reveals Its Essential Roles in Endocardial Cushion Formation, Cardiomyocyte Proliferation and Survival [J].
Chen, Li ;
Ma, Yanlin ;
Kim, Eun Young ;
Yu, Wei ;
Schwartz, Robert J. ;
Qian, Ling ;
Wang, Jun .
PLOS ONE, 2012, 7 (02)
[5]   The Pfam protein families database: towards a more sustainable future [J].
Finn, Robert D. ;
Coggill, Penelope ;
Eberhardt, Ruth Y. ;
Eddy, Sean R. ;
Mistry, Jaina ;
Mitchell, Alex L. ;
Potter, Simon C. ;
Punta, Marco ;
Qureshi, Matloob ;
Sangrador-Vegas, Amaia ;
Salazar, Gustavo A. ;
Tate, John ;
Bateman, Alex .
NUCLEIC ACIDS RESEARCH, 2016, 44 (D1) :D279-D285
[6]   Wheat mitogen-activated protein kinase gene TaMPK4 improves plant tolerance to multiple stresses through modifying root growth, ROS metabolism, and nutrient acquisitions [J].
Hao, Lin ;
Wen, Yanli ;
Zhao, Yuanyuan ;
Lu, Wenjing ;
Xiao, Kai .
PLANT CELL REPORTS, 2015, 34 (12) :2081-2097
[7]  
Jiang M, 2015, BMC GENOMICS, V16, DOI [10.1186/s12864-015-1452-1, 10.1186/s12864-015-1872-y]
[8]   Identification of mitogen-activated protein kinase kinase gene family and MKK-MAPK interaction network in maize [J].
Kong, Xiangpei ;
Pan, Jiaowen ;
Zhang, Dan ;
Jiang, Shanshan ;
Cai, Guohua ;
Wang, Li ;
Li, Dequan .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 441 (04) :964-969
[9]   WGCNA: an R package for weighted correlation network analysis [J].
Langfelder, Peter ;
Horvath, Steve .
BMC BIOINFORMATICS, 2008, 9 (1)
[10]   Clustal W and clustal X version 2.0 [J].
Larkin, M. A. ;
Blackshields, G. ;
Brown, N. P. ;
Chenna, R. ;
McGettigan, P. A. ;
McWilliam, H. ;
Valentin, F. ;
Wallace, I. M. ;
Wilm, A. ;
Lopez, R. ;
Thompson, J. D. ;
Gibson, T. J. ;
Higgins, D. G. .
BIOINFORMATICS, 2007, 23 (21) :2947-2948