Acute and chronic hypermethioninemia alter Na+,K+-ATPase activity in rat hippocampus: prevention by antioxidants

被引:19
作者
Stefanello, Francieli M. [1 ,2 ]
Ferreira, Andrea G. K. [1 ]
Pereira, Talita C. B. [3 ]
da Cunha, Maira J. [1 ]
Bohan, Carla D. [4 ]
Bogo, Mauricio R. [3 ]
Wyse, Angela T. S. [1 ]
机构
[1] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, Lab Neuroprotecao & Doenca Metab, BR-90035003 Porto Alegre, RS, Brazil
[2] Univ Fed Pelotas, Inst Quim & Geociencias, Dept Bioquim, Pelotas, RS, Brazil
[3] Pontificia Univ Catolica Rio Grande do Sul, Lab Biol Genom & Mol, Dept Biol Celular & Mol, Fac Biociencias, BR-90619900 Porto Alegre, RS, Brazil
[4] Pontificia Univ Catolica Rio Grande do Sul, Fac Biociencias, Dept Biol Celular & Mol, Lab Neuroquim & Psicofarmacol, BR-90619900 Porto Alegre, RS, Brazil
关键词
Hypermethioninemia; Na+; K+-ATPase; Vitamin E; Vitamin C; Hippocampus; Rat; SYNAPTIC PLASMA-MEMBRANE; ATPASE ALPHA-SUBUNIT; K+-ATPASE; VITAMIN-C; OXIDATIVE STRESS; ENERGY-METABOLISM; ASCORBIC-ACID; BRAIN; NA+; NA; K-ATPASE;
D O I
10.1016/j.ijdevneu.2011.02.001
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In the current study we initially investigated the influence of antioxidants (vitamins E plus C) on the effect mediated by acute and chronic administration of methionine (Met) on Na+,K+-ATPase activity in rat hippocampus. We also verified whether the alterations on the enzyme after administration of Met and/or antioxidants were associated with changes in relative expression of Na+,K+-ATPase catalytic sub-units (isoforms alpha 1, alpha 2 and alpha 3). For acute treatment, young rats received a single subcutaneous injection of Met or saline (control) and were sacrificed 12 h later. In another set of experiments, rats were pre-treated for 1 week with daily intraperitoneal administration of vitamins E (40 mg/kg) and C (100 mg/kg) or saline. After that, rats received a single injection of Met or saline and were killed 12 h later. For chronic treatment, Met was administered to rats from the 6th to the 28th day of life: controls and treated rats were sacrificed 12 h after the last injection. In parallel to chronic treatment, rats received a daily intraperitoneal injection of vitamins E and C from the 6th to the 28th day of life and were killed 12 h after the last injection. Results showed that administration of antioxidants partially prevented the inhibition of enzyme activity caused by acute and chronic hypermethioninemia. Besides, we demonstrated that transcription of catalytic subunits of Na+,K+-ATPase was not altered by chronic and acute exposure to Met and/or vitamins E plus C. These data strongly suggest the oxidative damage as one possible mechanism involved in the reduction of Na+,K+-ATPase activity caused by hypermethioninemia and if confirmed in human beings, we might propose the use of antioxidants as an adjuvant therapy in hypermethioninemic patients. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:483 / 488
页数:6
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