G1 cell cycle arrest signaling in hepatic injury after intraperitoneal sepsis in rats

被引：8

作者：

Yang, Quan-hui ^{[1
]}

Liu, Da-wei ^{[1
]}

Wang, Xiao-ting ^{[1
]}

Yang, Rong-li ^{[1
]}

Shi, Yan ^{[1
]}

Long, Yun ^{[1
]}

Liu, Hong-zhong ^{[1
]}

He, Huai-wu ^{[1
]}

Zhou, Xiang ^{[1
]}

Tang, Bo ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Crit Care Med, Beijing 100730, Peoples R China

来源：

INFLAMMATION RESEARCH | 2011年 / 60卷 / 08期

基金：

中国博士后科学基金;

关键词：

Liver; Cyclin; Cyclin-dependent kinase; Cyclin-dependent kinase inhibitor; SYSTEMIC INFLAMMATORY RESPONSE; LIVER-REGENERATION; ORGAN DYSFUNCTION; DEPENDENT KINASES; INTERLEUKIN-6-DEFICIENT MICE; LABORATORY MODELS; NUCLEAR ANTIGEN; SEPTIC SHOCK; IN-VIVO; FAILURE;

D O I：

10.1007/s00011-011-0334-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Hepatocytes emerge from a quiescent state into a proliferative state to recover from septic injury. We hypothesize that hepatocyte cell cycle regulation after sepsis potentially contributes to the recovery of liver function. An animal model of sepsis was induced by cecal ligation and puncture (CLP) in rats. At serial time points after CLP, hepatocyte expression of p21, P53, cyclin D1, cyclin E, CDK2, CDK4 and PCNA was determined by immunoblot analysis, and the DNA content of isolated hepatocytes was analyzed using flow cytometry. Sepsis-induced liver injury of rats was associated with G1 cell cycle arrest. Recovery of liver function was related to cell cycle progression 48 h after CLP. The upregulation of p53 and p21 correlated with G1 cell arrest 48 h after CLP. The upregulation of cyclin D1/CDK4 and cyclin E/CDK2 also correlated with the G1/S transition 48 h after CLP, resulting in PCNA expression. The data suggests that G1 cell cycle arrest and p53, p21, CDKs, cyclins and PCNA expression may be involved in the injury/recovery of liver function after intraperitoneal sepsis.

引用

页码：783 / 789

页数：7

共 48 条

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