Application of bacteriocin-producing Enterococcus faecium isolated from donkey milk, in the bio-control of Listeria monocytogenes in fresh whey cheese

被引：69
作者：
Aspri, Maria [1 ]
O'Connor, Paula M. [4 ]
Field, Des [2 ]
Cotter, Paul D.
Ross, Paul [3 ]
Hill, Colin [2 ,3 ]
Papademas, Photis [1 ]
机构：
[1] Cyprus Univ Technol, Dept Agr Sci Biotechnol & Food Sci, Limassol, Cyprus
[2] Univ Coll Cork, Dept Microbiol, Cork, Ireland
[3] TEAGASC, Agr & Food Dev Author, Cork, Ireland
[4] TEAGASC, Moorepk Food Res Ctr, Fermoy, Cork, Ireland
来源：
INTERNATIONAL DAIRY JOURNAL | 2017年 / 73卷
关键词：
LACTOCOCCUS-LACTIS; GENETIC-CHARACTERIZATION; DAIRY-PRODUCTS; STRAIN; PURIFICATION; EXPRESSION; SPECTRUM; STARTER; AS-48; FOOD;
D O I：
10.1016/j.idairyj.2017.04.008
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A total of 79 bacterial strains, previously isolated from donkey milk, were screened for their antimicrobial activity against several spoilage and foodborne pathogenic bacteria. Amongst them, 3 strains belonging to Enterococcus faecium displayed antimicrobial activity against Listeria monocytogenes, Staphylococcus aureus and Bacillus cereus. Mass spectrometry analysis demonstrated that all enterococci used in this study produced peptides with masses consistent with those for enterocins A and B. The cell-free supernatants of the identified bacteriocin-producing enterococci were equally active over a wide range of pH and heat treatments making them excellent candidates for potential applications in bio-preservation. Bacteriocins produced by these strains were tested for their capability to control post-processing contamination and growth of L. monocytogenes during refrigerated storage of artificially contaminated fresh whey cheese. One strain was considered bactericidal while the other two were classified as bacteriostatic. (C) 2017 Elsevier Ltd. All rights reserved.
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页码：1 / 9
页数：9
相关论文
共 47 条
[1]   Donkey milk: An overview on functionality, technology, and future prospects [J].
Aspri, Maria ;
Economou, Nicole ;
Papademas, Photis .
FOOD REVIEWS INTERNATIONAL, 2017, 33 (03) :316-333
[2]   Raw donkey milk as a source of Enterococcus diversity: Assessment of their technological properties and safety characteristics [J].
Aspri, Maria ;
Bozoudi, Despina ;
Tsaltas, Dimitris ;
Hill, Colin ;
Papademas, Photis .
FOOD CONTROL, 2017, 73 :81-90
[3]   Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins [J].
Aymerich, T ;
Holo, H ;
Havarstein, LS ;
Hugas, M ;
Garriga, M ;
Nes, IF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (05) :1676-1682
[4]   Evaluation of technological properties of Enterococcus faecium CECT 8849, a strain isolated from human milk, for the dairy industry [J].
Cardenas, Nivia ;
Arroyo, Rebeca ;
Calzada, Javier ;
Peiroten, Angela ;
Medina, Margarita ;
Miguel Rodriguez, Juan ;
Fernandez, Leonides .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (17) :7665-7677
[5]   Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A [J].
Casaus, P ;
Nilsen, T ;
Cintas, LM ;
Nes, IF ;
Hernandez, PE ;
Holo, H .
MICROBIOLOGY-SGM, 1997, 143 :2287-2294
[6]   Characteristics of bacteriocin-like inhibitory substances from dochi-isolated Enterococcus faecium D081821 and D081833 [J].
Chen, Y. -S. ;
Yanagida, F. ;
Srionnual, S. .
LETTERS IN APPLIED MICROBIOLOGY, 2007, 44 (03) :320-325
[7]   Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum [J].
Cintas, LM ;
Casaus, P ;
Havarstein, L ;
Hernandez, PE ;
Nes, IF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (11) :4321-4330
[8]   Real-Time PCR Assay To Differentiate Listeriolysin S-Positive and -Negative Strains of Listeria monocytogenes [J].
Clayton, Evelyn M. ;
Hill, Colin ;
Cotter, Paul D. ;
Ross, R. Paul .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (01) :163-171
[9]   Description of the bacteriocins produced by two strains of Enterococcus faecium isolated from Italian goat milk [J].
Cocolin, Luca ;
Foschino, Roberto ;
Comi, Giuseppe ;
Fortina, Maria Grazia .
FOOD MICROBIOLOGY, 2007, 24 (7-8) :752-758
[10]   Control of Listeria monocytogenes in fresh cheese using protective lactic acid bacteria [J].
Coelho, M. C. ;
Silva, C. C. G. ;
Ribeiro, S. C. ;
Dapkevicius, M. L. N. E. ;
Rosa, H. J. D. .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2014, 191 :53-59
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