Efficient bioremediation of PAHs-contaminated soils by a methylotrophic enrichment culture

被引:15
|
作者
Dhar, Kartik [1 ]
Panneerselvan, Logeshwaran [1 ,2 ]
Venkateswarlu, Kadiyala [3 ]
Megharaj, Mallavarapu [1 ,2 ]
机构
[1] Univ Newcastle, Global Ctr Environm Remediat GCER, Coll Engn Sci & Environm, ATC Bldg,Univ Dr, Callaghan, NSW 2308, Australia
[2] Univ Newcastle, Cooperat Res Ctr Contaminat Assessment & Remediat, ATC Bldg, Callaghan, NSW 2308, Australia
[3] Sri Krishnadevaraya Univ, Dept Microbiol, Anantapuramu 515003, India
关键词
Polycyclic aromatic hydrocarbons (PAHs); Bioremediation; Methylotrophic bacteria; Enrichment culture; Rhizobiaceae; Soil slurry; POLYCYCLIC AROMATIC-HYDROCARBONS; EX-SITU BIOREMEDIATION; MICROBIAL-DEGRADATION; PYRENE DEGRADATION; DIMETHYL-SULFOXIDE; SP-NOV; BACTERIA; BIODEGRADATION; BIOAUGMENTATION; IDENTIFICATION;
D O I
10.1007/s10532-022-09996-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bioaugmentation effectively enhances microbial bioremediation of hazardous polycyclic aromatic hydrocarbons (PAHs) from contaminated environments. While screening for pyrene-degrading bacteria from a former manufactured gas plant soil (MGPS), the mixed enrichment culture was found to be more efficient in PAHs biodegradation than the culturable pure strains. Interestingly, analysis of 16S rRNA sequences revealed that the culture was dominated by a previously uncultured member of the family Rhizobiaceae. The culture utilized C1 and other methylotrophic substrates, including dimethylformamide (DMF), which was used as a solvent for supplementing the culture medium with PAHs. In the liquid medium, the culture rapidly degraded phenanthrene, pyrene, and the carcinogenic benzo(a)pyrene (BaP), when provided as the sole carbon source or with DMF as a co-substrate. The efficiency of the culture in the bioremediation of PAHs from the MGPS and a laboratory waste soil (LWS) was evaluated in bench-scale slurry systems. After 28 days, 80% of Sigma 16 PAHs were efficiently removed from the inoculated MGPS. Notably, the bioaugmentation achieved 90% removal of four-ringed and 60% of highly recalcitrant five- and six-ringed PAHs from the MGPS. Likewise, almost all phenanthrene, pyrene, and 65% BaP were removed from the bioaugmented LWS. This study highlights the application of the methylotrophic enrichment culture dominated by an uncultured bacterium for the efficient bioremediation of PAHs.
引用
收藏
页码:575 / 591
页数:17
相关论文
共 50 条
  • [1] Efficient bioremediation of PAHs-contaminated soils by a methylotrophic enrichment culture
    Kartik Dhar
    Logeshwaran Panneerselvan
    Kadiyala Venkateswarlu
    Mallavarapu Megharaj
    Biodegradation, 2022, 33 : 575 - 591
  • [2] Efficient bioremediation of laboratory wastewater co-contaminated with PAHs and dimethylformamide by a methylotrophic enrichment culture
    Dhar, Kartik
    Sivaram, Anithadevi Kenday
    Panneerselvan, Logeshwaran
    Venkateswarlu, Kadiyala
    Megharaj, Mallavarapu
    JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2023, 325
  • [3] Enhanced anoxic bioremediation of PAHs-contaminated sediment
    Lu, Xiao-Ying
    Li, Bing
    Zhang, Tong
    Fang, Herbert H. P.
    BIORESOURCE TECHNOLOGY, 2012, 104 : 51 - 58
  • [4] Burkholderia fungorum DBT1: a promising bacterial strain for bioremediation of PAHs-contaminated soils
    Andreolli, Marco
    Lampis, Silvia
    Zenaro, Elena
    Salkinoja-Salonen, Mirja
    Vallini, Giovanni
    FEMS MICROBIOLOGY LETTERS, 2011, 319 (01) : 11 - 18
  • [5] Effects of compost stability and contaminant concentration on the bioremediation of PAHs-contaminated soil through composting
    Sayara, Tahseen
    Sarra, Montserrat
    Sanchez, Antoni
    JOURNAL OF HAZARDOUS MATERIALS, 2010, 179 (1-3) : 999 - 1006
  • [6] Bioremediation of PAHs-contaminated soil through composting: Influence of bioaugmentation and biostimulation on contaminant biodegradation
    Sayara, Tahseen
    Borras, Eduard
    Caminal, Gloria
    Sarra, Montserrat
    Sanchez, Antoni
    INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2011, 65 (06) : 859 - 865
  • [7] Effects of different carbon substrates on PAHs fractions and microbial community changes in PAHs-contaminated soils
    Wang, Jinfeng
    Zhang, He
    Cai, Jun
    Li, Jia
    Sun, Benhua
    Wu, Fuyong
    ENVIRONMENTAL POLLUTION, 2023, 324
  • [8] Enhanced PAHs-contaminated site soils remediation by mixed persulfate and calcium peroxide
    Tang, Lei
    Zhang, Xiaochun
    Li, Zekai
    Gudda, Fredrick Owino
    Waigi, Michael Gatheru
    Wang, Jian
    Liu, Hua
    Gao, Yanzheng
    JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2022, 306
  • [9] Dynamics of microbial community and functional genes during bioremediation of PAHs-contaminated soil by two biostimulants
    Chen, Tao
    Dong, Yanli
    Huang, Wenbiao
    Ma, Yilin
    BIOCHEMICAL ENGINEERING JOURNAL, 2024, 208
  • [10] Bioremediation of Soils Contaminated with polycyclic aromatic Hydrocarbons (PAHs): A Review
    Li, Yi
    Wang, Hongqi
    2012 4TH INTERNATIONAL CONFERENCE ON ENVIRONMENTAL SCIENCE AND INFORMATION APPLICATION TECHNOLOGY (ESIAT 2012), 2013, 14 : 166 - 173