Efficient bioremediation of PAHs-contaminated soils by a methylotrophic enrichment culture

被引:15
|
作者
Dhar, Kartik [1 ]
Panneerselvan, Logeshwaran [1 ,2 ]
Venkateswarlu, Kadiyala [3 ]
Megharaj, Mallavarapu [1 ,2 ]
机构
[1] Univ Newcastle, Global Ctr Environm Remediat GCER, Coll Engn Sci & Environm, ATC Bldg,Univ Dr, Callaghan, NSW 2308, Australia
[2] Univ Newcastle, Cooperat Res Ctr Contaminat Assessment & Remediat, ATC Bldg, Callaghan, NSW 2308, Australia
[3] Sri Krishnadevaraya Univ, Dept Microbiol, Anantapuramu 515003, India
关键词
Polycyclic aromatic hydrocarbons (PAHs); Bioremediation; Methylotrophic bacteria; Enrichment culture; Rhizobiaceae; Soil slurry; POLYCYCLIC AROMATIC-HYDROCARBONS; EX-SITU BIOREMEDIATION; MICROBIAL-DEGRADATION; PYRENE DEGRADATION; DIMETHYL-SULFOXIDE; SP-NOV; BACTERIA; BIODEGRADATION; BIOAUGMENTATION; IDENTIFICATION;
D O I
10.1007/s10532-022-09996-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bioaugmentation effectively enhances microbial bioremediation of hazardous polycyclic aromatic hydrocarbons (PAHs) from contaminated environments. While screening for pyrene-degrading bacteria from a former manufactured gas plant soil (MGPS), the mixed enrichment culture was found to be more efficient in PAHs biodegradation than the culturable pure strains. Interestingly, analysis of 16S rRNA sequences revealed that the culture was dominated by a previously uncultured member of the family Rhizobiaceae. The culture utilized C1 and other methylotrophic substrates, including dimethylformamide (DMF), which was used as a solvent for supplementing the culture medium with PAHs. In the liquid medium, the culture rapidly degraded phenanthrene, pyrene, and the carcinogenic benzo(a)pyrene (BaP), when provided as the sole carbon source or with DMF as a co-substrate. The efficiency of the culture in the bioremediation of PAHs from the MGPS and a laboratory waste soil (LWS) was evaluated in bench-scale slurry systems. After 28 days, 80% of Sigma 16 PAHs were efficiently removed from the inoculated MGPS. Notably, the bioaugmentation achieved 90% removal of four-ringed and 60% of highly recalcitrant five- and six-ringed PAHs from the MGPS. Likewise, almost all phenanthrene, pyrene, and 65% BaP were removed from the bioaugmented LWS. This study highlights the application of the methylotrophic enrichment culture dominated by an uncultured bacterium for the efficient bioremediation of PAHs.
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页码:575 / 591
页数:17
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