Comparable CD4 and CD8 T cell responses and cytokine release after at-birth and delayed BCG immunisation in infants born in Australia

被引:11
作者
Ritz, Nicole [1 ,2 ,3 ,4 ]
Casalaz, Dan [5 ]
Donath, Susan [6 ]
Tebruegge, Marc [1 ,2 ,3 ,7 ,8 ]
Dutta, Binita [3 ]
Connell, Tom G. [1 ,2 ,3 ]
Robins-Browne, Roy [1 ,2 ,3 ,9 ]
Britton, Warwick J. [10 ,11 ]
Hanekom, Willem A. [12 ,13 ]
Curtis, Nigel [1 ,2 ,3 ]
机构
[1] Univ Melbourne, Dept Paediat, Parkville, Vic, Australia
[2] Royal Childrens Hosp Melbourne, Infect Dis Unit, Parkville, Vic, Australia
[3] Royal Childrens Hosp, Murdoch Childrens Res Inst, Infect Dis & Microbiol Grp, Parkville, Vic, Australia
[4] Univ Basel, Childrens Hosp Basel, Infect Dis Unit & Paediat Pharmacol, Basel, Switzerland
[5] Mercy Hosp Women, Dept Paediat, Heidelberg, Australia
[6] Royal Childrens Hosp Melbourne, Murdoch Childrens Res Inst, Clin Epidemiol & Biostat Unit, Parkville, Vic, Australia
[7] Univ Southampton, Fac Med, Acad Unit Clin & Expt Sci, Southampton, Hants, England
[8] Univ Southampton, Inst Life Sci, Southampton, Hants, England
[9] Univ Melbourne, Peter Doherty Inst Infect & Immun, Dept Microbiol & Immunol, Melbourne, Vic, Australia
[10] Univ Sydney, Centenary Inst Canc Med & Cell Biol, Camperdown, NSW, Australia
[11] Univ Sydney, Dept Med, Camperdown, NSW, Australia
[12] Univ Cape Town, Inst Infect Dis & Mol Med, South African TB Vaccine Initiat, Cape Town, South Africa
[13] Univ Cape Town, Dept Paediat & Child Hlth, Cape Town, South Africa
基金
英国医学研究理事会;
关键词
Bacille Calmette-Guerin; HIV; Immune maturation; Vaccine; Tuberculosis; TB; CALMETTE-GUERIN VACCINE; IMMUNE-RESPONSE; TUBERCULOUS MENINGITIS; 4; CONTINENTS; CHILDREN; AGE; NEWBORNS; DISEASE;
D O I
10.1016/j.vaccine.2016.06.077
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background: More than 120 million doses of BCG vaccine are administered worldwide each year. Most infants are given BCG at birth in accordance with WHO recommendations. However, the effect of the maturing neonatal immune system on the immune response and protection conferred by BCG remains uncertain. Previous studies investigating the influence of age at immunisation on the immune response induced by BCG have reported conflicting results. This study compared BCG given at birth and at two months of age in infants in Australia. Methods: Infants born in Melbourne were randomly allocated to immunisation with BCG-Denmark at birth or two months of age. Ten weeks after immunisation, anti-mycobacterial immune responses were measured in a whole blood assay using intracellular cytokine assays and xMAP multiplex cytokine analysis. Results: Result from 98 BCG-immunised infants were included in the final analysis. BCG immunisation at birth (n = 54) and at 2 months of age (n = 44) induced comparable proportions of mycobacteria-specific cytokine-producing CD4 and CD8 T cells, as well as comparable proportions of polyfunctional (TNF+ IL-2(+) IFN-gamma(+)) CD4 T cells. Concentrations of cytokines in supernatants were also similar in both groups. Conclusions: Cellular immunity measured 10 weeks after BCG immunisation was similar in infants given BCG at birth and in those given BCG at 2 months of age. Although definitive correlates of protection against TB remain uncertain, these results suggest that delaying BCG immunisation does not confer any immunological advantage in cellular immunity. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4132 / 4139
页数:8
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