African 2, a Clonal Complex of Mycobacterium bovis Epidemiologically Important in East Africa

被引：73

作者：

Berg, Stefan ^{[1
]}

Garcia-Pelayo, M. Carmen ^{[1
]}

Mueller, Borna ^{[2
]}

Hailu, Elena ^{[3
]}

Asiimwe, Benon ^{[4
]}

Kremer, Kristin ^{[5
]}

Dale, James ^{[1
]}

Boniotti, M. Beatrice ^{[6
]}

Rodriguez, Sabrina ^{[7
,8
]}

Hilty, Markus ^{[9
]}

Rigouts, Leen ^{[19
]}

Firdessa, Rebuma ^{[3
]}

Machado, Adelina ^{[18
]}

Mucavele, Custodia ^{[18
]}

Ngandolo, Bongo Nare Richard ^{[14
]}

Bruchfeld, Judith ^{[11
,12
]}

Boschiroli, Laura ^{[6
,10
]}

Mueller, Annelle ^{[2
]}

Sahraoui, Naima ^{[16
]}

Pacciarini, Maria ^{[6
]}

Cadmus, Simeon ^{[13
]}

Joloba, Moses ^{[4
]}

van Soolingen, Dick ^{[5
]}

Michel, Anita L. ^{[20
,21
]}

Djonne, Berit ^{[17
]}

Aranaz, Alicia ^{[7
,8
]}

Zinsstag, Jakob ^{[23
]}

van Helden, Paul ^{[2
]}

Portaels, Francoise ^{[19
]}

Kazwala, Rudovick ^{[15
]}

Kallenius, Gunilla ^{[22
]}

Hewinson, R. Glyn ^{[1
]}

Aseffa, Abraham ^{[3
]}

Gordon, Stephen V. ^{[24
,25
]}

Smith, Noel H. ^{[1
,26
]}

机构：

[1] VLA Weybridge, Surrey KT15 3NB, England

[2] Univ Stellenbosch, Div Mol Biol & Human Genet, Fac Hlth Sci, ZA-7505 Tygerberg, South Africa

[3] Armauer Hansen Res Inst, Addis Ababa, Ethiopia

[4] Makerere Univ, Dept Med Microbiol, Sch Med, Kampala, Uganda

[5] Ctr Infect Dis Control CIb LIS, Natl Inst Publ Hlth & Environm RIVM, TB Reference Lab, NL-3720 BA Bilthoven, Netherlands

[6] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Reparto Genom, I-25124 Brescia, Italy

[7] Univ Complutense, Dept Sanidad Anim, Fac Vet, E-28040 Madrid, Spain

[8] Univ Complutense, Ctr Vigilancia Sanitaria Vet VISAVET, E-28040 Madrid, Spain

[9] Univ Bern, Inst Infect Dis, CH-3010 Bern, Switzerland

[10] Agence Francaise Securite Sanit Aliments, F-94706 Maisons Alfort, France

[11] Dept Med, Infect Dis Unit, Solna, Sweden

[12] Karolinska Univ Hosp, Karolinska Inst, S-17177 Stockholm, Sweden

[13] Univ Ibadan, Dept Vet Publ Hlth & Prevent Med, Ibadan, Nigeria

[14] Lab Rech Vet & Zootechn Farcha, Ndjamena, Chad

[15] Sokoine Univ Agr, Morogoro, Tanzania

[16] Univ Saad Dahlab, Blida, Algeria

[17] Natl Vet Inst, Dept Anim Hlth, N-0106 Oslo, Norway

[18] Univ Eduardo Mondlane, Fac Vet, Maputo, Mozambique

[19] Inst Trop Med, Dept Microbiol, B-2000 Antwerp, Belgium

[20] Univ Pretoria, Fac Vet Sci, ZA-0110 Onderstepoort, South Africa

[21] ARC Onderstepoort Vet Inst, ZA-0110 Onderstepoort, South Africa

[22] Karolinska Inst, Dept Clin Sci & Educ, S-11883 Stockholm, Sweden

[23] Swiss Trop & Publ Hlth Inst, CH-4002 Basel, Switzerland

[24] Univ Coll Dublin, Coll Life Sci, Dublin 4, Ireland

[25] Univ Coll Dublin, UCD Conway Inst, Dublin 4, Ireland

[26] Univ Sussex, Ctr Study Evolut, Brighton BN1 9QL, E Sussex, England

来源：

JOURNAL OF BACTERIOLOGY | 2011年 / 193卷 / 03期

基金：

瑞士国家科学基金会; 瑞典研究理事会; 英国医学研究理事会; 新加坡国家研究基金会; 英国惠康基金;

关键词：

FRAGMENT-LENGTH-POLYMORPHISM; DIRECT REPEAT LOCUS; TUBERCULOSIS COMPLEX; MOLECULAR EPIDEMIOLOGY; STRAIN DIFFERENTIATION; GENETIC-MARKERS; SPOLIGOTYPE DIVERSITY; EVOLUTIONARY SCENARIO; INSERTION ELEMENTS; DNA POLYMORPHISM;

D O I：

10.1128/JB.00750-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110, in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.

引用

页码：670 / 678

页数：9

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