Chemically induced coalescence in droplet-based microfluidics

被引:59
作者
Akartuna, Ilke [1 ]
Aubrecht, Donald M. [1 ]
Kodger, Thomas E. [1 ]
Weitz, David A. [1 ]
机构
[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
基金
美国国家科学基金会;
关键词
ELECTROPORATION; FLOW; ELECTROCOALESCENCE; TENSION;
D O I
10.1039/c4lc01285b
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We present a new microfluidic method to coalesce pairs of surfactant-stabilized water-in-fluorocarbon oil droplets. We achieve this through the local addition of a poor solvent for the surfactant, perfluorobutanol, which induces cohesion between droplet interfaces causing them to merge. The efficiency of this technique is comparable to existing techniques providing an alternative method to coalesce pairs of droplets.
引用
收藏
页码:1140 / 1144
页数:5
相关论文
共 29 条
[1]   Ultrahigh-throughput screening in drop-based microfluidics for directed evolution [J].
Agresti, Jeremy J. ;
Antipov, Eugene ;
Abate, Adam R. ;
Ahn, Keunho ;
Rowat, Amy C. ;
Baret, Jean-Christophe ;
Marquez, Manuel ;
Klibanov, Alexander M. ;
Griffiths, Andrew D. ;
Weitz, David A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (09) :4004-4009
[2]   Electrocoalescence of drops synchronized by size-dependent flow in microfluidic channels [J].
Ahn, Keunho ;
Agresti, Jeremy ;
Chong, Henry ;
Marquez, Manuel ;
Weitz, D. A. .
APPLIED PHYSICS LETTERS, 2006, 88 (26)
[3]   Formation of dispersions using "flow focusing" in microchannels [J].
Anna, SL ;
Bontoux, N ;
Stone, HA .
APPLIED PHYSICS LETTERS, 2003, 82 (03) :364-366
[4]   Surfactants in droplet-based microfluidics [J].
Baret, Jean-Christophe .
LAB ON A CHIP, 2012, 12 (03) :422-433
[5]   An optical toolbox for total control of droplet microfluidics [J].
Baroud, Charles N. ;
de Saint Vincent, Matthieu Robert ;
Delville, Jean-Pierre .
LAB ON A CHIP, 2007, 7 (08) :1029-1033
[6]   Decompressing emulsion droplets favors coalescence [J].
Bremond, Nicolas ;
Thiam, Abdou R. ;
Bibette, Jerome .
PHYSICAL REVIEW LETTERS, 2008, 100 (02)
[7]   Droplet microfluidic technology for single-cell high-throughput screening [J].
Brouzes, Eric ;
Medkova, Martina ;
Savenelli, Neal ;
Marran, Dave ;
Twardowski, Mariusz ;
Hutchison, J. Brian ;
Rothberg, Jonathan M. ;
Link, Darren R. ;
Perrimon, Norbert ;
Samuels, Michael L. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (34) :14195-14200
[8]   DIRECT MEASUREMENT OF INTERFACIAL INTERACTIONS BETWEEN SEMISPHERICAL LENSES AND FLAT SHEETS OF POLY(DIMETHYLSILOXANE) AND THEIR CHEMICAL DERIVATIVES [J].
CHAUDHURY, MK ;
WHITESIDES, GM .
LANGMUIR, 1991, 7 (05) :1013-1025
[9]   Microfluidic electroporation for cellular analysis and delivery [J].
Geng, Tao ;
Lu, Chang .
LAB ON A CHIP, 2013, 13 (19) :3803-3821
[10]   Droplet microfluidics for high-throughput biological assays [J].
Guo, Mira T. ;
Rotem, Assaf ;
Heyman, John A. ;
Weitz, David A. .
LAB ON A CHIP, 2012, 12 (12) :2146-2155