Novel fluorescent dyes based on oligopropylamines for the in vivo staining of eukaryotic unicellular algae

被引:33
作者
Annenkov, V. V. [1 ]
Danilovtseva, E. N. [1 ]
Zelinskiy, S. N. [1 ]
Basharina, T. N. [1 ]
Safonova, T. A. [1 ]
Korneva, E. S. [1 ]
Likhoshway, Y. V. [1 ]
Grachev, M. A. [1 ]
机构
[1] Russian Acad Sci, Limnol Inst, Siberian Branch, Irkutsk 660033, Russia
关键词
Fluorescent dye; Oligopropylamines; Eukaryote; Diatom; Silica deposition vesicle; ORGANIC MICROCRYSTALS; AMINO-ACIDS; SILICA; NANOPARTICLES; DIATOMS; NBD; PHOSPHOLIPIDOSIS; MODULATION; DEPOSITION;
D O I
10.1016/j.ab.2010.07.032
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Weakly basic fluorescent dyes are used to visualize organelles within live cells due to their affinity to acidic subcellular organelles. In particular, they are used to stain the silica deposited in the silica deposition vesicles (SDVs) of diatoms during the course of their frustule synthesis. This study involved the synthesis of fluorescent dyes derived from oligopropylamines, compounds similar to those found in diatoms. The dyes were obtained by reacting oligopropylamines with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. The reaction was realized using methylated oligopropylamines with two or three nitrogen atoms and yielded two novel fluorescent dyes: NBD-N2 and NBD-N3. The dyes appeared to be highly efficient in the in vivo staining of growing siliceous frustules of diatoms at concentrations at least 10 times lower than those required for staining with HCK-123. NBD-N3 also efficiently stained other subcellular vesicles of eukaryotic unicellular algae. NBD-N2 stained only growing diatom frustules, whereas NBD-N3 also stained various subcellular organelles of different eukaryotic unicellular algae. NBD-N2 and NBD-N3 were not removed from stained diatom frustules by drastic treatments with H2SO4 and H2O2. Fluorescent silica can also be obtained by its chemical precipitation in the presence of NBD-N2 and NBD-N3. (C) 2010 Elsevier Inc. All rights reserved.
引用
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页码:44 / 51
页数:8
相关论文
共 45 条
  • [1] ABYAN M, 2006, INT J PHOTOENERGY, V8, P1
  • [2] Suspensions of Organic Microcrystals Produced in the Presence of Polymers: Diversity of UV/Vis Absorption and Fluorescence Properties According to the Preparation Conditions
    Abyan, Mouhammad
    de Caro, Dominique
    Fery-Forgues, Suzanne
    [J]. LANGMUIR, 2009, 25 (03) : 1651 - 1658
  • [3] Biomineralization at the Nanoscale: Learning from Diatoms
    Allison, David P.
    Dufrene, Yves F.
    Doktycz, Mitchel J.
    Hildebrand, Mark
    [J]. METHODS IN NANO CELL BIOLOGY, 2008, 90 : 61 - 86
  • [4] Synthesis of biomimetic polyamines
    Annenkov, Vadim V.
    Zelinskiy, Stanislav N.
    Danilovtseva, Elena N.
    Perry, Carole C.
    [J]. ARKIVOC, 2009, : 116 - 130
  • [5] A new stepwise synthesis of a family of propylamines derived from diatom silaffins and their activity in silicification
    Annenkov, VV
    Patwardhan, SV
    Belton, D
    Danilovtseva, EN
    Perry, CC
    [J]. CHEMICAL COMMUNICATIONS, 2006, (14) : 1521 - 1523
  • [6] The life of diatoms in the world's oceans
    Armbrust, E. Virginia
    [J]. NATURE, 2009, 459 (7244) : 185 - 192
  • [7] Bioinspired Silicification of Functional Materials: Fluorescent Monodisperse Mesostructure Silica Nanospheres
    Begum, Gousia
    Rana, Rohit K.
    Singh, Shashi
    Satyanarayana, L.
    [J]. CHEMISTRY OF MATERIALS, 2010, 22 (02) : 551 - 556
  • [8] Synthesis and fluorescent properties of new derivatives of 4-amino-7-nitrobenzofurazan
    Bem, Marioara
    Badea, Florin
    Draghici, Constantin
    Caproiu, Miron T.
    Vasilescu, Marilena
    Voicescu, Mariana
    Beteringhe, Adrian
    Caragheorgheopol, Agneta
    Maganu, Maria
    Constantinescu, Titus
    Balaban, Alexandru T.
    [J]. ARKIVOC, 2007, : 87 - 104
  • [9] Dendrimer-tuned formation of fluorescent organic microcrystals. Influence of dye hydrophobicity and dendrimer charge
    Bertorelle, Franck
    Rodrigues, Fernanda
    Fery-Forgues, Suzanne
    [J]. LANGMUIR, 2006, 22 (20) : 8523 - 8531
  • [10] Distinct pathways of antigen uptake and intracellular routing in CD4 and CD8 T cell activation
    Burgdorf, Sven
    Kautz, Andreas
    Boehnert, Volker
    Knolle, Percy A.
    Kurts, Christian
    [J]. SCIENCE, 2007, 316 (5824) : 612 - 616