Effect of triamcinolone acetonide on proliferation of retinal endothelial cells in vitro and in vivo

被引:29
作者
Spandau, UHM
Sauder, G
Schubert, U
Hammes, HP
Jonas, JB
机构
[1] Fac Clin Med, Dept Ophthalmol, Mannheim, Germany
[2] Univ Heidelberg, Heidelberg, Germany
[3] Fac Clin Med, Dept Internal Med, Mannheim, Germany
[4] Univ Giessen, Inst Biochem, Giessen, Germany
关键词
D O I
10.1136/bjo.2004.052563
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Aim: To assess the effect of crystalline triamcinolone acetonide on retinal endothelial cell proliferation in vivo and in vitro. Methods: For in vitro analysis, a sprouting assay was employed. Bovine retinal endothelial cells were stimulated with basic fibroblast growth factor (bFGF) and incubated with different concentrations of triamcinolone acetonide (0.05 mg/ml to 8 mg/ml). For in vivo analysis, a retinopathy of prematurity (ROP) model was used. 16 C57BL/J6 mice were exposed to 75% oxygen from postnatal day 7 to day 12. On day 12, triamcinolone acetonide was intravitreally injected into one eye ("study eye") and isotonic saline into the contralateral eye ("control eye"). On day 17, the mice were sacrificed and the eyes removed for quantitative analysis of preretinal neovascularisation. Four non-exposed mice served as negative control. Results: The sprouting assay demonstrated a dose dependent inhibition of bovine retinal endothelial cell proliferation from 0.05 mg triamcinolone acetonide/ml (no inhibition) to 3 mg triamcinolone acetonide/ml (complete inhibition). Dosages of more than 2 mg/ml resulted in cytotoxic changes of endothelial cells. The ROP model demonstrated a significantly lower neovascular cell count of 58% in the study group compared to the control group (6.35 (SD 2.1) cells per histological section versus 14.9 (SD 5.3) cells; p < 0.005). Conclusions: Triamcinolone acetonide inhibits bFGF induced proliferation of retinal endothelial cells in vivo and in vitro. These findings contribute to understanding the mode of action and effects of triamcinolone acetonide on retinal neovascularisation.
引用
收藏
页码:745 / 747
页数:3
相关论文
共 50 条
[31]   Retinal angiomatous proliferation: combined therapy of intravitreal triamcinolone acetonide and PDT versus PDT alone [J].
Krebs, Ilse ;
Krepler, Katharina ;
Stolba, Ulrike ;
Goll, Alexandra ;
Binder, Susanne .
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 2008, 246 (02) :237-243
[32]   Retinal angiomatous proliferation: combined therapy of intravitreal triamcinolone acetonide and PDT versus PDT alone [J].
Ilse Krebs ;
Katharina Krepler ;
Ulrike Stolba ;
Alexandra Goll ;
Susanne Binder .
Graefe's Archive for Clinical and Experimental Ophthalmology, 2008, 246 :237-243
[33]   Toxicity of triamcinolone acetonide (Kenalog) and preservative-free triamcinolone acetonide on cultured human retinal pigment epithelial (ARPE-19) cells [J].
Marques, LA ;
Narayanan, R ;
Luthra, S ;
Kenney, MC ;
Kuppermann, BD .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2005, 46
[34]   Effect of triamcinolone acetonide on retinal inflammation and angiogenesis induced by pericyte depletion in mouse [J].
Otsuka, Tomohiro ;
Masuda, Tomomi ;
Takahashi, Yuji ;
Suzuki, Ayako ;
Uemura, Akiyoshi ;
Arakawa, Reijiro ;
Okabe, Takeshi ;
Naito, Akira .
JOURNAL OF PHARMACOLOGICAL SCIENCES, 2023, 151 (01) :28-36
[35]   Effect of hypoxia on the proliferation of retinal microvessel endothelial cells in culture [J].
Lou, YQ ;
Oberpriller, JC ;
Carlson, EC .
ANATOMICAL RECORD, 1997, 248 (03) :366-373
[36]   In vitro permeation studies of triamcinolone acetonide mouthwashes [J].
Ungphaiboon, S ;
Maitani, Y .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 220 (1-2) :111-117
[37]   Polymeric triamcinolone acetonide nanoparticles as a new alternative in the treatment of uveitis: In vitro and in vivo studies [J].
Sabzevari, Araz ;
Adibkia, Khosro ;
Hashemi, Hassan ;
Hedayatfar, Alireza ;
Mohsenzadeh, Navid ;
Atyabi, Fatemeh ;
Ghahremani, Mohammad Hossein ;
Dinarvand, Rassoul .
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2013, 84 (01) :63-71
[38]   Effects of intravitreal triamcinolone acetonide on diabetic retinal neovascularization [J].
Ben Yahia, S ;
Zeghidi, H ;
Attia, S ;
Zaouali, S ;
Ladjimi, A ;
Khairallah, M .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 :U374-U374
[39]   Retinal break following an intravitreal triamcinolone acetonide injection [J].
Erol N. ;
Topbas S. ;
Sahin A. .
International Ophthalmology, 2005, 26 (4-5) :163-165
[40]   Retinal toxicity of intravitreal triamcinolone acetonide; a morphologic study [J].
Yu, SY ;
Viola, F ;
Damico, FM ;
D'Amico, DJ .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 :U745-U745