Colocalization of transcription and translation within cytoplasmic poxvirus factories coordinates viral expression and subjugates host functions

被引:207
作者
Katsafanas, George C. [1 ]
Moss, Bernard [1 ]
机构
[1] Natl Inst Hlth, Natl Inst Allergy & Infect Dis, Viral Dis Lab, Bethesda, MD 20892 USA
关键词
D O I
10.1016/j.chom.2007.08.005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Poxviruses are large DNA viruses that include the causal agent of human smallpox and vaccinia virus. Poxviruses replicate in cytoplasmic foci known as DNA factories. Here we show that a virus-encoded transcription factor, viral mRNA, cellular RNA-binding protein heterodimer G3BP/Caprin-1 (p137), translation initiation factors elF4E and elF4G, and ribosomal proteins are concentrated in the same sub-domains of cytoplasmic DNA factories. Furthermore, a cell coinfected with two recombinant vaccinia viruses expressing a virus core protein fused to cyan or yellow fluorescent protein displayed separate cyan and yellow factories, indicating that each factory formed from a single genome and was the site of transcription and translation as well as DNA replication. Hijacking of the host translation apparatus within the factory likely enhances the efficiency of virus replication and contributes to the suppression of host protein synthesis, thereby facilitating poxvirus subjugation of the cell.
引用
收藏
页码:221 / 228
页数:8
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