Listeria monocytogenes biofilm inhibition on food contact surfaces by application of postbiotics from Lactobacillus curvatus B.67 and Lactobacillus plantarum M.2

被引：81

作者：

Hossain, Md Iqbal ^{[1
]}

Mizan, Md Furkanur Rahaman ^{[1
]}

Roy, Pantu Kumar ^{[1
]}

Nahar, Shamsun ^{[1
]}

Toushik, Sazzad Hossen ^{[1
]}

Ashrafudoulla, Md ^{[1
]}

Jahid, Iqbal Kabir ^{[2
]}

Lee, Jihyun ^{[3
]}

Ha, Sang-Do ^{[1
]}

机构：

[1] Chung Ang Univ, Dept Food Sci & Technol, Adv Food Safety Res Grp, 72-1 Nae Ri, Anseong 456756, Gyunggido, South Korea

[2] Jashore Univ Sci & Technol, Dept Microbiol, Jashore, Bangladesh

[3] Chung Ang Univ, Dept Food Sci & Technol, Anseong 17546, South Korea

来源：

FOOD RESEARCH INTERNATIONAL | 2021年 / 148卷

基金：

新加坡国家研究基金会;

关键词：

Probiotic; Postbiotic; Metabolite; Listeria monocytogenes; Biofilm; Food contact surface; Food industry; LACTIC-ACID BACTERIA; CLASS IIA BACTERIOCIN; IN-VITRO; ANTIMICROBIAL ACTIVITY; 2-PEPTIDE BACTERIOCIN; ANTIBIOTIC-RESISTANCE; ANTAGONISTIC ACTIVITY; ESCHERICHIA-COLI; ESSENTIAL OILS; VIRULENCE;

D O I：

10.1016/j.foodres.2021.110595

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Owing to their preservative and antimicrobial effects, postbiotics (metabolic byproducts of probiotics) are promising natural components for the food industry. Therefore, the present study aimed to investigate the efficacy of postbiotics collected from isolated Lactobacillus curvatus B.67 and Lactobacillus plantarum M.2 against Listeria monocytogenes pathogens in planktonic cells, motility, and biofilm states. The analysis of the metabolite composition of the postbiotics revealed various organic acids, along with a few well-known bacteriocin-encoding genes with potential antimicrobial effects. Postbiotics maintained their residual antimicrobial activity over the pH range 1-6 but lost all activity at neutral pH (pH 7). Full antimicrobial activity (100%) was observed during heat treatment, even under the autoclaving condition. Minimum inhibitory concentration (MICs) of L. curvatus B.67 and L. plantarum M.2 against L. monocytogenes were 80 and 70 mg/mL, respectively. However, four subMICs of the postbiotics (1/2, 1/4, 1/8, and 1/16 MIC) were tested for inhibition efficacy against L. monocytogenes during different experiment in this study. Swimming motility, biofilm formation, and expression levels of target genes related to biofilm formation, virulence, and quorum-sensing were significantly inhibited with increasing postbiotics concentration. Postbiotics from L. plantarum M.2 exhibited a higher inhibitory effect than the postbiotics from L. curvatus B.67. Nonetheless, both these postbiotics from Lactobacillus spp. could be used as effective bio-interventions for controlling L. monocytogenes biofilm in the food industry.

引用

页数：13

共 81 条

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