Embryotoxicity of cobalt ferrite and gold nanoparticles: A first in vitro approach

被引：63
作者：
Di Guglielmo, Claudia [1 ]
Ramos Lopez, David [1 ]
De Lapuente, Joaquin [1 ]
Llobet Mallafre, Joan Maria [2 ]
Borras Suarez, Miguel [1 ]
机构：
[1] UTOX PCB, Unit Expt Toxicol & Ecotoxicol, Barcelona 08028, Spain
[2] Univ Barcelona, GRET CERETOX, Toxicol Unit, Dept Publ Hlth,Fac Pharm, Barcelona, Spain
来源：
REPRODUCTIVE TOXICOLOGY | 2010年 / 30卷 / 02期
关键词：
Mouse embryonic stem cells test; Teratology; Embryotoxicity; Gold nanoparticles; Cobalt ferrite nanoparticles; Cytotoxicity; Differentiation; STEM-CELL TEST; DEVELOPMENTAL TOXICITY; DIFFERENTIATION; CYTOTOXICITY; PARTICLES; LINES; ASSAY;
D O I：
10.1016/j.reprotox.2010.05.001
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Nanoparticles (NPs) are emerging as promising biomedical tools thanks to their peculiar characteristics. Our purpose was to investigate the embryotoxicity of cobalt ferrite and gold NPs through the Embryonic Stem Cell Test (EST). The EST is an in vitro standard assay, which permits to classify substances as strongly, weakly or non-embryotoxic. Due to the particular physical-chemical nature of nanoparticles, we introduced a modification to the standard protocol exposing the Embryonic Stem Cells (ES-D3) to nanoparticles only during the first 5 days of the assay. Moreover, we proposed a method to discriminate and compare the embryotoxicity of the substances within the weakly embryotoxic range. Our ID50 results permit to classify cobalt ferrite nanoparticles coated with gold and silanes as non-embryotoxic. The remaining nanoparticles have been classified as weakly embryotoxic in this decreasing order: gold salt (HAuCl4 center dot 3H(2)O) > cobalt ferrite salt (CoFe2O4) > cobalt ferrite nanoparticles coated with silanes (Si-CoFe) > gold nanoparticles coated with hyaluronic acid (HA-Au). (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：271 / 276
页数：6
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