Antioxydant and Hepatoprotective effect of aqueous extract of Pistacia atlantica Desf on Rat's exposed to Mercury

The purpose of the present study is to evaluate hepato-protective effect of natural products of aqueous extract of Pistacia atlantica against oxidative stress induced by toxicity of mercuric chloride in liver of male rats. In the first time, determination of total phenolic and flavonoid contents as well as the antioxidant activity of the leaves extract was carried out. In the second part, Adult Wistar rats weighing between 55 and 65 g were randomly assigned to 3 lots. The animals were administered with mercury chloride (HgCl2) intraperitoneally for 32 days. The group intoxicated receive 2.5 mg/kg of HgCl2, while the treated group receive 2.5 mg/kg of HgCl2 and 150 mg/kg of P. atlantica. The tested extract showed a significant antioxidant properties and high phenolic and flavonoid contents. In our experimentation, we obtained a significant change in enzymatic activity. We also observed the significantly a decrease in hepatic glutathione levels, the enzymatic activity of antioxidant system GST and the expression of lipid peroxidation by a higher level of hepatic MDA. In addition, supplementation by aqueous extract of Pistacia atlantica modify the toxic effects of mercury by improving somes disturbances. This results show that the administration of aqueous extract of P. atlantica is a good extract with very promising therapeutic values for the treatment of liver damages