Analysis of the virus population present in equine faeces indicates the presence of hundreds of uncharacterized virus genomes

被引：79

作者：

Cann, AJ ^{[1
]}

Fandrich, SE ^{[1
]}

Heaphy, S ^{[1
]}

机构：

[1] Univ Leicester, Dept Infect Immun & Inflammat, Leicester LE1 9HN, Leics, England

来源：

VIRUS GENES | 2005年 / 30卷 / 02期

关键词：

bacteriophage genomes; equine; gene discovery;

D O I：

10.1007/s11262-004-5624-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Virus DNA was isolated from horse faeces and cloned in a sequence- independent fashion. 268 clones were sequenced and 178140 nucleotides of sequence obtained. Statistical analysis suggests the library contains 17560 distinct clones derived from up to 233 different virus genomes. TBLASTX analysis showed that 32% of the clones had significant identity to GenBank entries. Of these 63% were viral; 20% bacterial; 7% archaeal; 6% eukarya; and 5% were related to mobile genetic elements. Fifty-two percent of the virus identities were with Siphoviridae; 26% unclassified phages; 17% Myoviridae; 4% Podoviridae; and one clone (2%) was a vertebrate Orthopoxvirus. Genes coding for predicted virus structural proteins, proteases, glycosidases and nucleic acid-binding proteins were common.

引用

页码：151 / 156

页数：6

共 16 条

[1] Bacteriophage-bacteriophage interactions in the evolution of pathogenic bacteria [J].

Boyd, EF ;

Davis, BM ;

Hochhut, B .

TRENDS IN MICROBIOLOGY, 2001, 9 (03) :137-144

[2] Metagenomic analyses of an uncultured viral community from human feces [J].

Breitbart, M ;

Hewson, I ;

Felts, B ;

Mahaffy, JM ;

Nulton, J ;

Salamon, P ;

Rohwer, F .

JOURNAL OF BACTERIOLOGY, 2003, 185 (20) :6220-6223

[3] Genomic analysis of uncultured marine viral communities [J].

Breitbart, M ;

Salamon, P ;

Andresen, B ;

Mahaffy, JM ;

Segall, AM ;

Mead, D ;

Azam, F ;

Rohwer, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (22) :14250-14255

[4] Prophages and bacterial genomics: what have we learned so far? [J].

Casjens, S .

MOLECULAR MICROBIOLOGY, 2003, 49 (02) :277-300

[5]

CHAO A, 1984, SCAND J STAT, V11, P265

[6]

Daly K, 2001, FEMS MICROBIOL ECOL, V38, P141, DOI 10.1111/j.1574-6941.2001.tb00892.x

[7] Environmental diversity of Bacteria and Archaea [J].

DeLong, EE ;

Pace, NR .

SYSTEMATIC BIOLOGY, 2001, 50 (04) :470-478

[8] Marine viruses and their biogeochemical and ecological effects [J].

Fuhrman, JA .

NATURE, 1999, 399 (6736) :541-548

[9] Bacteriophage genomics [J].

Hendrix, RW .

CURRENT OPINION IN MICROBIOLOGY, 2003, 6 (05) :506-511

[10] Unexpected diversity of small eukaryotes in deep-sea Antarctic plankton [J].

López-García, P ;

Rodríguez-Valera, F ;

Pedrós-Alió, C ;

Moreira, D .

NATURE, 2001, 409 (6820) :603-607

← 1 2 →