Suitability of selective media for recovery of heat-stressed Escherichia coli O157:H7

被引:5
|
作者
Fisher, TL [1 ]
Golden, DA [1 ]
机构
[1] Univ Tennessee, Dept Food Sci & Technol, Agr Expt Stn, Knoxville, TN 37901 USA
来源
JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY | 1998年 / 6卷 / 03期
关键词
D O I
10.1111/j.1745-4581.1998.tb00200.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Tryptone soya agar (TSA) and three selective media; BCM(TM) O157:H7(+) agar (BCM), modified eosin methylene blue agar (MEMB), and sorbitol MacConkey agar (SMAC) were evaluated for recovery of two strains off. coli O157:H7 (salami and cider isolates) heated at 56 58, and 60C for up to 60 min in tryptone soya broth (TSB). TSA and MEMB were equally effective at recovery of heat-stressed (56 58, and 60C) E. coli O157:H7 and superior to SMAC and BCM (P less than or equal to 0.05). When heated at 56 and 58C, recovery off. coli O157:H7 on MEMB and TSA was not significantly different (P > 0.05); recovery was poorer an SMAC, followed by BCM(P less than or equal to 0.05). There was no significant difference in recovery off. coli O157:H7 on BCM and SMAC when strains were heated at 60C (P > 0.05).
引用
收藏
页码:211 / 218
页数:8
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