ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle

被引：7

作者：

Tay, Darren Dean ^{[1
]}

Siew, Shing Wei ^{[1
]}

Shamzir Kamal, Shamrulazhar ^{[1
]}

Razali, Mohd Najib ^{[2
,3
]}

Ahmad, Hajar Fauzan ^{[1
,4
]}

机构：

[1] Univ Malaysia Pahang, Fac Ind Sci & Technol, Lebuhraya Tun Razak, Gambang 26300, Pahang, Malaysia

[2] Univ Malaysia Pahang, Fac Chem & Proc Engn Technol, Lebuhraya Tun Razak, Gambang 26300, Pahang, Malaysia

[3] MNR Multitech Sdn Bhd, UMP Holdings Complex, Lebuhraya Tun Razak, Gambang 26300, Pahang, Malaysia

[4] Ctr Res Adv Trop Biosci Biotrop Ctr, Lebuhraya Tun Razak, Gambang 26300, Pahang, Malaysia

来源：

ARCHIVES OF MICROBIOLOGY | 2022年 / 204卷 / 06期

关键词：

Mycobiome; Fungi; Flongle; Feline gut; Malaysia; MICROBIOTA; DIDYMOSPHAERIACEAE; PENIOPHORELLA; INFECTION; HEALTH; WORLD; CATS; DOGS;

D O I：

10.1007/s00203-022-02929-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gut mycobiome exhibits major influence on the gastrointestinal health and disease but received less attention due to low abundance. This study characterizes the fungal community and compares the microbial diversity between indoor and outdoor cats. Genomic DNA was extracted and sequenced by targeting the Internal Transcribed Spacer 1 (ITS1) region using Flongle flow cell on MinION (TM) sequencing platform. Results show the phylum Ascomycota and genus Peniophorella were numerous in indoor cats, whereas the Basidiomycota and Pichia were abundant in outdoor cats. Peniophorella formed the core mycobiome in both feline populations. Furthermore, alpha (p value = 0.0207) and beta diversities (p value = 0.009) results showed significant differences between the two groups. Overall, indoor cats have greater amounts of Peniophorella, whereas outdoor cats have higher Trichosporon and unclassified Sordariaceae. The study also suggests that keeping a cat indoors or left as a stray will affect their respective gut mycobiome.

引用

页数：11

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