CELLULAR DIFFERENTIATION EXACERBATES RADIATION SENSITIVITY IN VITRO IN A HUMAN DOPAMINERGIC NEURONAL MODEL

被引：0
作者：
Temelie, M. [1 ,2 ]
Mustaciosu, C. [1 ]
Flonta, M. L. [2 ]
Savu, D. [1 ]
机构：
[1] Horia Hulubei Natl Inst Phys & Nucl Engn, Dept Life & Environm Phys, Magurele, Romania
[2] Univ Bucharest, Dept Anat Anim Physiol & Biophys, Fac Biol, 91-95 Splaiul Independentei, Bucharest, Romania
来源：
ROMANIAN REPORTS IN PHYSICS | 2017年 / 69卷 / 03期
关键词：
UV; DNA damage; differentiation; neurons; SH-SY5Y; SH-SY5Y NEUROBLASTOMA-CELLS; HEMATOPOIETIC STEM-CELLS; DNA-DAMAGE RESPONSE; STRAND BREAK REPAIR; APOPTOSIS; CANCER; CYCLE; MODULATION; MECHANISMS; EXPRESSION;
D O I：
暂无
中图分类号：
O4 [物理学];
学科分类号：
0702 ;
摘要：
UV radiations are well known cellular stressors. Following exposure to genotoxic factors the cells respond by molecular mechanisms aimed to repair the damages, block the proliferation or trigger cell death. Our Study investigates how neuronal differentiation interferes with repair processes following UV -induced genotoxic stress, in a model of dopaminergic neurons (SH-SY5Y). Our work proved a higher level of DNA damage induced by UVC radiation in the dose range of 0.3582.150 mJ/cm(2) in differentiated cells compared to the proliferative variants. Cellular viability decreases proportionally with the irradiation dose and post-exposure time and correlates with an increased apoptosis through caspase 3/7 activation. These effects were enhanced in differentiated cells in which we have seen increased DNA damage, incomplete repair, exacerbated viability loss and apoptosis induction.
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页数：15
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