Validated RP-HPLC Method Development for Gentamicin Microanalysis in Pharmaceutical Dosage Forms, Urine and Blood

被引：7

作者：

Maheshwari, M. L. ^{[1
,2
]}

Memon, N. ^{[1
]}

Memon, A. A. ^{[1
]}

Khuhawer, M. Y. ^{[3
]}

Memon, A. H. ^{[4
]}

机构：

[1] Univ Sindh, Natl Ctr Excellence Analyt Chem, Jamshoro, Pakistan

[2] Univ Sindh, Dept Pharmaceut Chem, Fac Pharm, Jamshoro, Pakistan

[3] Univ Sindh, Inst Adv Res Studies Chem Sci, Jamshoro, Pakistan

[4] Univ Sindh, Fac Pharm, Dept Pharmacognosy, Jamshoro, Pakistan

来源：

PHARMACEUTICAL CHEMISTRY JOURNAL | 2021年 / 55卷 / 05期

关键词：

Gentamicin; Hantzsch reagent; derivatization; HPLC-PDA; PERFORMANCE LIQUID-CHROMATOGRAPHY; PRECOLUMN DERIVATIZATION; SULFATE; ASSAY; SERUM;

D O I：

10.1007/s11094-021-02451-8

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Gentamicin is an important member of the aminoglycoside class of antibiotics having broader spectrum with extend to ototoxicity and nephrotoxcity. A reliable and accurate high performance liquid chromatography (HPLC) procedure was established using Agilent Zorbax Eclipse plus phenyl hexyl C18 (4.6 x 250 mm, 5 mu m) column and UV detection at 330 nm. Recovery of gentamicin from biological and pharmaceutical samples was more than 95-98% after spiking the drug. The sensitivity of method is predicted by calculating a lower level of the limit of detection and lower limit of quantification (0.286 and 0.867 mu g/mL, respectively. The procedure was used to quantify gentamicin in biological fluids and pharmaceuticals dosage form.

引用

页码：524 / 530

页数：7

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