Unbalanced phospholipid composition of Escherichia coli membranes affects PPHO promoter activity

被引：2
|
作者：
Golovastov, VV [1 ]
Krasovskaya, LA
Anissimova, EV
Shchukin, VN
Badyakina, AO
Nesmeyanova, MA
机构：
[1] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorgan, Pushchino 142290, Moscow Region, Russia
[2] Samara State Univ, Samara 443011, Russia
来源：
MOLECULAR BIOLOGY | 2005年 / 39卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
promoter; beta-galactosidase; expression; phosphatidylethanolamine; anionic phospholipids; membrane sensor; Escherichia coli;
D O I：
10.1007/s11008-005-0037-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The activity of phosphatase P-PHO or arabinose P-BAD promoters of Escherichia coli has been studied as dependent on the content of zwitterionic phosphatidylethanolamine (PE) and anionic phospholipids in membranes. In the absence of PE or upon a significant decrease in the content of anionic phospholipids, the activity of the P-PHO promoter (but not that of the P-BAD promoter) was inhibited. Since the P-PHO promoter belongs to the Pho regulon, a member of the family of two-component regulatory systems of signal transduction that have membrane sensors, the regulation of gene expression by phospholipids is presumed to be accomplished through a membrane sensor.
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页码：260 / 268
页数：9
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