De Novo Glycine Synthesis Is Reduced in Adults With Morbid Obesity and Increases Following Bariatric Surgery

被引:17
作者
Tan, Hong Chang [1 ]
Hsu, Jean W. [2 ,3 ]
Tai, E. Shyong [4 ]
Chacko, Shaji [2 ,3 ]
Wu, Vieon [1 ]
Lee, Chun Fan [5 ]
Kovalik, Jean-Paul [6 ]
Jahoor, Farook [2 ,3 ]
机构
[1] Singapore Gen Hosp, Dept Endocrinol, Singapore, Singapore
[2] Baylor Coll Med, Childrens Nutr Res Ctr, US Dept Agr, Agr Res Serv, Houston, TX USA
[3] Baylor Coll Med, Dept Pediat, Houston, TX USA
[4] Natl Univ Hlth Syst, Yong Loo Lin Sch Med, Dept Med, Singapore, Singapore
[5] Duke NUS Med Sch, Ctr Quantitat Med, Singapore, Singapore
[6] Duke NUS Med Sch, Cardiovasc & Metab Disorders Program, Singapore, Singapore
来源
FRONTIERS IN ENDOCRINOLOGY | 2022年 / 13卷
基金
英国医学研究理事会;
关键词
morbid obesity; bariatric surgery; de novo glycine synthesis; insulin resistancE; stable-isotope tracers; metabolic flux; substrate kinetics; glycine; BETA-CELL FUNCTION; INSULIN SENSITIVITY; AMINO-ACIDS; PROTEIN-METABOLISM; IN-VIVO; TURNOVER; GLUCOSE; HOMEOSTASIS; RESISTANCE; LEUCINE;
D O I
10.3389/fendo.2022.900343
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
BackgroundGlycine is a dietary non-essential amino acid that is low in obesity and increases following bariatric surgery. However, the exact mechanism responsible remains unclear and it is unknown whether hypoglycinemia is a cause or consequence of insulin resistance. ObjectiveUsing multiple isotopically labeled tracers, we aimed to determine the underlying kinetic changes responsible for hypoglycinemia in obesity by: 1) Comparing glycine kinetics between participants with morbid obesity (BMI >= 32.5 kg/m(2)) to those with healthy weight (BMI < 25 kg/m(2)), and 2) Comparing glycine kinetic changes in participants with morbid obesity after bariatric surgery. Methods[1,2-C-13(2)] glycine, [2,3,3-H-2(3)] serine, and [H-2(5)] phenylalanine were infused to compare the glycine kinetic parameters between 21 participants with morbid obesity and 21 controls with healthy weight. Participants with morbid obesity then underwent bariatric surgery and 17 were re-studied 6 months later. Data were analyzed by non-parametric methods and presented as median (interquartile range). ResultsCompared to controls, participants with morbid obesity had significantly lower plasma glycine concentrations at 163 (153-171) vs. 201 (172-227) mu mol/L and significantly reduced de novo glycine synthesis rate at 86.2 (64.5-111) vs.124 (103-159) mu mol center dot kg LBM-1 center dot h(1), p < 0.001. Following surgery, body weight and insulin resistance decreased and this was accompanied by significant increases in plasma glycine concentration to 210 (191-243) mu mol/L as well as the de novo glycine synthesis rate to 127 (98.3-133) mu mol center dot kg LBM-1 center dot h(-1), p < 0.001 vs. baseline. ConclusionHypoglycinemia in participants with morbid obesity was associated with impaired de novo glycine synthesis. The increase in plasma glycine concentration and de novo glycine synthesis plus the marked improvement in insulin resistance after bariatric surgery suggest that hypoglycinemia may be secondary to impaired glycine synthesis because of obesity-induced insulin resistance.
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页数:14
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