Microfluidic single-cell transcriptomics: moving towards multimodal and spatiotemporal omics

被引:35
作者
Lin, Shichao [1 ]
Liu, Yilong [1 ]
Zhang, Mingxia [1 ]
Xu, Xing [1 ]
Chen, Yingwen [1 ]
Zhang, Huimin [1 ,2 ]
Yang, Chaoyong [1 ,2 ,3 ]
机构
[1] Xiamen Univ, MOE Key Lab Spectrochem Anal & Instrumentat, State Key Lab Phys Chem Solid Surfaces, Key Lab Chem Biol Fujian Prov,Dept Chem Biol,Coll, Xiamen 361005, Peoples R China
[2] Innovat Lab Sci & Technol Energy Mat Fujian Prov, Xiamen 361005, Peoples R China
[3] Shanghai Jiao Tong Univ, Inst Mol Med, Renji Hosp, Sch Med, Shanghai 200127, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划; 中国博士后科学基金;
关键词
GENOME-WIDE EXPRESSION; HIGH-THROUGHPUT; DIGITAL MICROFLUIDICS; GENE-EXPRESSION; TUMOR-CELLS; LOW-COST; RNA-SEQ; DIELECTROPHORESIS; NUMBER; TISSUE;
D O I
10.1039/d1lc00607j
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cells are the basic units of life with vast heterogeneity. Single-cell transcriptomics unveils cell-to-cell gene expression variabilities, discovers novel cell types, and uncovers the critical roles of cellular heterogeneity in biological processes. The recent advances in microfluidic technologies have greatly accelerated the development of single-cell transcriptomics with regard to throughput, sensitivity, cost, and automation. In this article, we review state-of-the-art microfluidic single-cell transcriptomics, with a focus on the methodologies. We first summarize six typical microfluidic platforms for isolation and transcriptomic analysis of single cells. Then the on-going trend of microfluidic transcriptomics towards multimodal omics, which integrates transcriptomics with other omics to provide more comprehensive pictures of gene expression networks, is discussed. We also highlight single-cell spatial transcriptomics and single-cell temporal transcriptomics that provide unprecedented spatiotemporal resolution to reveal transcriptomic dynamics in space and time, respectively. The emerging applications of microfluidic single-cell transcriptomics are also discussed. Finally, we discuss the current challenges to be tackled and provide perspectives on the future development of microfluidic single-cell transcriptomics.
引用
收藏
页码:3829 / 3849
页数:21
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