Application of polymerase chain reaction for early detection of infectious bursal disease virus infection

被引：0

作者：

Parthiban, M ^{[1
]}

Thiagarajan, V ^{[1
]}

机构：

[1] Tamil Nadu Vet & Anim Sci Univ, Chennai 600007, Tamil Nadu, India

来源：

INDIAN JOURNAL OF ANIMAL SCIENCES | 2000年 / 70卷 / 12期

关键词：

agar-gel precipitation test; dot-enzyme linked immunosorbent assay; infectious bursal disease virus; reverse transcription-polymerase chain reaction;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The bursae suspensions at different hours past - infection were subjected tc AG PT, dot-E LISA and RT-PCR to compare their sensitivity in detecting the presence of IBD virus in the early stage of infection. Until 36 hr post - infection, the antigen could not be detected by AGPT and dot-ELISA, whereas it was possible to detect viral RNA by RT-PCR at 24 hr post - infection. Hence, RT-PCR was highly sensitive in detecting the virus during early stage of IBDV infection.

引用

页码：1201 / 1203

页数：3

共 16 条

[1] THE CHARACTERIZATION AND MOLECULAR-CLONING OF THE DOUBLE-STRANDED-RNA GENOME OF AN AUSTRALIAN STRAIN OF INFECTIOUS BURSAL DISEASE VIRUS [J].

AZAD, AA ;

BARRETT, SA ;

FAHEY, KJ .

VIROLOGY, 1985, 143 (01) :35-44

[2]

Becht H, 1980, Curr Top Microbiol Immunol, V90, P107

[3] BIOPHYSICAL AND BIOCHEMICAL CHARACTERIZATION OF 5 ANIMAL VIRUSES WITH BISEGMENTED DOUBLE-STRANDED-RNA GENOMES [J].

DOBOS, P ;

HILL, BJ ;

HALLETT, R ;

KELLS, DTC ;

BECHT, H ;

TENINGES, D .

JOURNAL OF VIROLOGY, 1979, 32 (02) :593-605

[4] INFECTIVITY OF INFECTIOUS BURSAL DISEASE VIRUS FOR EMBRYONATING EGGS [J].

HITCHNER, SB .

POULTRY SCIENCE, 1970, 49 (02) :511-+

[5] CHARACTERISTICS AND SEROLOGIC STUDIES OF 2 SEROTYPES OF INFECTIOUS BURSAL DISEASE VIRUS IN TURKEYS [J].

JACKWOOD, DJ ;

SAIF, YM ;

HUGHES, JH .

AVIAN DISEASES, 1982, 26 (04) :871-882

[6] SEQUENCE COMPARISONS OF A HIGHLY VIRULENT INFECTIOUS BURSAL DISEASE VIRUS PREVALENT IN JAPAN [J].

LIN, Z ;

KATO, A ;

OTAKI, Y ;

NAKAMURA, T ;

SASMAZ, E ;

UEDA, S .

AVIAN DISEASES, 1993, 37 (02) :315-323

[7] DETECTION OF GENETIC VARIATIONS IN SEROTYPE-I ISOLATES OF INFECTIOUS BURSAL-DISEASE VIRUS USING POLYMERASE CHAIN-REACTION AND RESTRICTION-ENDONUCLEASE ANALYSIS [J].

LIU, HJ ;

GIAMBRONE, JJ ;

DORMITORIO, T .

JOURNAL OF VIROLOGICAL METHODS, 1994, 48 (2-3) :281-291

[8] AN IMPROVED DOT ELISA TO DETECT FOWL ADENOVIRUS TYPE-1 ANTIGEN [J].

MAITI, NK ;

SAINI, SS ;

SINGH, R ;

OBEROI, MS ;

SHARMA, SN .

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 1993, 16 (03) :245-250

[9]

NACHIMUTHU K, 1993, INDIAN J ANIM SCI, V63, P817

[10]

QIAN B, 1994, J VIROL METHODS, V47, P337

← 1 2 →