Mass-tag technology for monitoring of protein kinase activity using mass spectrometry

被引：12

作者：

Sonoda, T

Shigaki, S

Nagashima, T

Okitsu, O

Kita, Y

Murata, M

Katayama, Y ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Higashi Ku, Fukuoka 8128581, Japan

[2] Fujisawa Pharmaceut Co Ltd, Adv Technol Platform Lab, Tsukuba, Ibaraki 3002698, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2004年 / 14卷 / 04期

关键词：

protein kinases; protein phosphorylation; high-throughput screening; mass spectrometry;

D O I：

10.1016/j.bmcl.2003.12.022

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using P-32, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities. (C) 2003 Elsevier Ltd. All rights reserved.

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页码：847 / 850

页数：4

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